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Invitrogen
CHD4 Recombinant Rabbit Monoclonal Antibody (0M3R5)
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FIGURE: 1 / 3
CHD4 Antibody (MA5-55408) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Human spleen using CHD4 Recombinant Monoclonal Antibody (Product # MA5-55408) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6. 0) prior to IHC staining.
Product Details
MA5-55408
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0M3R5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1700 of human CHD4.
Conjugate
Form
Liquid
Concentration
1.22 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667649
Product Specific Information
Immunogen Sequence: ELAEVEENKK MSQPGSPSPK TPTPSTPGDT QPNTPAPVPP AEDGIKIEEN SLKEEESIEG EKEVKSTAPE TAIECTQAPA PASEDEKVVV EPPEGEEKVE KAEVKERTEE PMETEPKGAA DVEKVEEKSA IDLTPIVVED KEEKKEEEEK KEVMLQNGET PKDLNDEKQK KNIKQRFMFN I
Target Information
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase CHD4; CHD-4; Chromodomain-helicase-DNA-binding protein 4; Mi-2 autoantigen; Mi-2 autoantigen 218 kDa protein; Mi-2 beta; Mi2-beta; MI2B
Gene Aliases: 9530019N15Rik; AA617397; BC005710; CHD-4; CHD4; D6Ertd380e; Mi-2; Mi-2b; Mi-2beta; Mi2-BETA; mKIAA4075
UniProt ID: (Human) Q14839, (Mouse) Q6PDQ2
Entrez Gene ID: (Human) 1108, (Mouse) 107932, (Rat) 117535
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II repressing transcription factor binding
DNA binding
ATP-dependent DNA helicase activity
histone deacetylase activity
protein binding
ATP binding
microtubule binding
zinc ion binding
nucleosomal DNA binding
nucleotide binding
nucleic acid binding
helicase activity
ATP-dependent helicase activity
transcription factor binding
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
metal ion binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
histone deacetylation
DNA duplex unwinding
ATP-dependent chromatin remodeling
spindle assembly
terminal button organization
regulation of signal transduction by p53 class mediator
chromatin organization
regulation of transcription, DNA-templated
chromatin modification
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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