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Invitrogen
CHD5 Polyclonal Antibody
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Product Details
PA5-37148
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to residues in human CHD5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2553931
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 230-250 kDa.
Purity is >95% by SDS-PAGE.
Target Information
CHD5 (Chromodomain helicase DNA binding protein 5) is a member of the SNF2/RAD54 helicase family of chromatin remodeling and DNA-binding proteins (CDH proteins). Heavily expressed in both fetal and adult brain, CHD5 plays a role in nervous system development and acts as a tumor suppressor via the Arf/p53 pathway. CHD5, along with other chromo-domain proteins, forms remodeling complexes, such as NuRD, that promote normal neuroblast maturation and are thought to prevent overexpression of neuronal cells. Errors in these chromatin remodeling complexes can leave the cell in a perpetual state of growth, preventing differentiation and leading to tumor formation. Due to the importance of the CHD proteins in proper brain development, deletions in the gene encoding CHD5 are commonly found in neuroblastomas, suggesting that CHD5 deficiency may lead to malignant cell transformation and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent helicase CHD5; CHD-5; CHD5; Chromodomain-helicase-DNA-binding protein 5
Gene Aliases: 4930532L22Rik; AW060752; B230399N07Rik; CHD-5; CHD5; KIAA0444
UniProt ID: (Human) Q8TDI0
Entrez Gene ID: (Human) 26038, (Mouse) 269610
DNA binding
ATP binding
ATP-dependent helicase activity
zinc ion binding
H3K27me3 modified histone binding
nucleotide binding
nucleic acid binding
chromatin binding
helicase activity
protein binding
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
histone deacetylase binding
metal ion binding
transcription, DNA-templated
negative regulation of cell proliferation
cerebral cortex neuron differentiation
spermatogenesis, exchange of chromosomal proteins
histone H4 acetylation
regulation of transcription involved in cell fate commitment
histone H3-K27 trimethylation
positive regulation of signal transduction by p53 class mediator
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
spermatogenesis
nervous system development
chromatin modification
cell differentiation
regulation of cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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