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Invitrogen
CHFR Polyclonal Antibody
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FIGURE: 1 / 5
CHFR Antibody (PA5-106807) in ICC/IF
Immunocytochemistry analysis of CHFR in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CHFR polyclonal antibody (Product # PA5-106807) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106807
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CHFR(Accession Q96EP1), corresponding to amino acid residues C344-V394.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854475
Product Specific Information
Antibody detects endogenous levels of total CHFR.
Target Information
E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Checkpoint with forkhead and RING finger domains protein; E3 ubiquitin-protein ligase CHFR; FLJ10796; FLJ33629; RING finger protein 196; RING-type E3 ubiquitin transferase CHFR
Gene Aliases: 5730484M20Rik; C230082M18; CHFR; RNF116; RNF196
UniProt ID: (Human) Q96EP1, (Mouse) Q810L3
Entrez Gene ID: (Human) 55743, (Rat) 288734, (Mouse) 231600
nucleotide binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
mitotic cell cycle
ubiquitin-dependent protein catabolic process
mitotic cell cycle checkpoint
protein ubiquitination
modification-dependent protein catabolic process
positive regulation of protein ubiquitination
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
mitotic nuclear division
cell division
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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