Proteintech

CHMP1A Polyclonal Antibody

CHMP1A Antibody in Immunocytochemistry (ICC/IF)

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CHMP1A Antibody (15761-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 15761-1-AP (CHMP1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CHMP1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP1A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15761-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHMP1A Fusion Protein Ag8389 (1-196 aa encoded by BC007527)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDDTLFQLK FTAKQLEKLA KKAEKDSKAE QAKVKKALLQ KNVECARVYA ENAIRKKNEG VNWLRMASRV DAVASKVQTA VTMKGVTKNM AQVTKALDKA LSTMDLQKVS SVMDRFEQQV QNLDVHTSVM EDSMSSATTL TTPQEQVDSL IMQIAEENGL EVLDQLSQLP EGASAVGESS VRSQEDQLSR RLAALRN (1-196 aa encoded by BC007527)

Target Information

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I, -II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation.

Journal of extracellular vesicles

Marie PP,Fan SJ,Mason J,Wells A,Mendes CC,Wainwright SM,Scott S,Fischer R,Harris AL,Wilson C,Goberdhan DCI

Wed Mar 01 00:00:00 UTC 2023

A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.

Nature communications

Guan Y,Liang X,Ma Z,Hu H,Liu H,Miao Z,Linkermann A,Hellwege JN,Voight BF,Susztak K

Mon Aug 23 00:00:00 UTC 2021

A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.

Nature communications

Guan Y,Liang X,Ma Z,Hu H,Liu H,Miao Z,Linkermann A,Hellwege JN,Voight BF,Susztak K

Mon Aug 23 00:00:00 UTC 2021

miR-17 regulates the proliferation and differentiation of retinal progenitor cells by targeting CHMP1A.

Biochemical and biophysical research communications

Sun N,Zhang D,Ni N,Tang Z,Gao H,Ju Y,Dai X,Wang J,Gu P,Ji J

Thu Mar 05 00:00:00 UTC 2020

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 UTC 2020

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Methods in molecular biology (Clifton, N.J.)

Adar-Levor S,Goliand I,Elbaum M,Elia N

Mon Mar 02 00:00:00 UTC 2020

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Cell reports

Coulter ME,Dorobantu CM,Lodewijk GA,Delalande F,Cianferani S,Ganesh VS,Smith RS,Lim ET,Xu CS,Pang S,Wong ET,Lidov HGW,Calicchio ML,Yang E,Gonzalez DM,Schlaeger TM,Mochida GH,Hess H,Lee WA,Lehtinen MK,Kirchhausen T,Haussler D,Jacobs FMJ,Gaudin R,Walsh CA

Tue Jul 24 00:00:00 UTC 2018

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Cell reports

Tue Jul 24 00:00:00 UTC 2018

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Cell reports

Tue Jul 24 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Nature communications

Scheffer LL,Sreetama SC,Sharma N,Medikayala S,Brown KJ,Defour A,Jaiswal JK

Tue Dec 23 00:00:00 UTC 2014

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Nature communications

Scheffer LL,Sreetama SC,Sharma N,Medikayala S,Brown KJ,Defour A,Jaiswal JK

Tue Dec 23 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: charged multivesicular body protein 1/chromatin modifying protein 1; Charged multivesicular body protein 1a; CHMP1a; chromatin modifying protein 1A; Chromatin-modifying protein 1a; hVps46-1; procollagen (type III) N-endopeptidase; protease, metallo, 1, 33kD; Vacuolar protein sorting-associated protein 46-1; Vps46-1

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Gene Aliases: 2900018H07Rik; CHMP1; CHMP1A; KIAA0047; PCH8; PCOLN3; PRSM1; VPS46-1; VPS46A

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UniProt ID: (Human) Q9HD42, (Mouse) Q921W0

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Entrez Gene ID: (Human) 5119, (Mouse) 234852

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Function(s)

protein binding metallopeptidase activity zinc ion binding protein domain specific binding identical protein binding protein homodimerization activity

Process(es)

cell separation after cytokinesis transcription, DNA-templated regulation of transcription, DNA-templated transport nucleus organization vacuolar transport cell cycle mitotic chromosome condensation mitotic metaphase plate congression regulation of centrosome duplication protein transport vesicle-mediated transport gene silencing negative regulation of transcription by glucose negative regulation of transcription, DNA-templated cell division regulation of mitotic spindle assembly proteolysis multivesicular body assembly viral budding via host ESCRT complex ESCRT III complex disassembly

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CHMP1A Polyclonal Antibody

CHMP1A Antibody (15761-1-AP) in ICC/IF

Product Details

15761-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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