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Invitrogen
CHMP3 Polyclonal Antibody
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FIGURE: 1 / 1
CHMP3 Antibody (PA5-87794) in WB
Western blot analysis of CHMP3 in extracts of various cell lines. Samples were incubated with CHMP3 Polyclonal antibody (Product # PA5-87794) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Product Details
PA5-87794
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human VPS24 (NP_0571631)
Conjugate
Form
Liquid
Concentration
2.29 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804415
Product Specific Information
Immunogen sequence: MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS; Positive Samples: BT-474, LO2, 293T, SW480; Cellular Location: Cytoplasm, Endosome, Late endosome membrane, Lipid-anchor, Membrane, cytosol
Target Information
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25.1 protein; Charged multivesicular body protein 3; CHMP family, member 3; Chromatin-modifying protein 3; hVps24; Neuroendocrine differentiation factor; vacuolar protein sorting 24; vacuolar protein sorting 24 homolog; Vacuolar protein sorting-associated protein 24; vacuolar protein-sorting-associated protein 24
Gene Aliases: 25.1; 4921505F14Rik; 9130011K15Rik; CGI-149; CGI-49; CGI149; CHMP3; D6Ertd286e; NEDF; VPS24
UniProt ID: (Human) Q9Y3E7, (Mouse) Q9CQ10
Entrez Gene ID: (Human) 51652, (Mouse) 66700
cell separation after cytokinesis
autophagy
apoptotic process
nucleus organization
mitotic metaphase plate congression
regulation of centrosome duplication
protein transport
endosomal transport
viral life cycle
multivesicular body assembly
viral budding via host ESCRT complex
regulation of viral process
protein polymerization
protein heterooligomerization
multivesicular body sorting pathway
autophagosome maturation
regulation of mitotic spindle assembly
negative regulation of viral release from host cell
positive regulation of viral release from host cell
regulation of exosomal secretion
transport
vacuolar transport
cell cycle
endosome to lysosome transport
positive regulation of cytokinesis
regulation of endosome size
cell division
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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