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Invitrogen
CHMP4A Polyclonal Antibody
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CHMP4A Antibody (PA5-49410) in WB
Western blot analysis of CHMP4A in lysates from human brain and human placenta tissue (from left to right). Samples were incubated with CHMP4A polyclonal antibody (Product # PA5-49410) using a dilution of 1:1,000 followed by goat anti-rabbit IgG H&L (HRP) at a dilution of 1:10,000. Lysates at 20 µg per lane.
Product Details
PA5-49410
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 205-239 amino acids from the C-terminal region of human CHMP4A.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2634864
Target Information
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Charged multivesicular body protein 4a; CHMP4a; chromatin modifying protein 4A; Chromatin-modifying protein 4a; hSnf-1; hVps32-1; SNF7 homolog associated with Alix-2; Snf7 homologue associated with Alix 2; SNF7-1; Vacuolar protein sorting-associated protein 32-1; Vps32-1
Gene Aliases: C14orf123; CDA04; CHMP4; CHMP4A; CHMP4B; HSPC134; SHAX2; SNF7; SNF7-1; VPS32-1; VPS32A
UniProt ID: (Human) Q9BY43
Entrez Gene ID: (Human) 29082
cell separation after cytokinesis
posttranslational protein targeting to membrane
membrane budding
autophagy
nucleus organization
mitotic metaphase plate congression
membrane invagination
endosomal transport
viral life cycle
multivesicular body assembly
viral budding via host ESCRT complex
protein polymerization
protein homooligomerization
membrane tubulation
negative regulation of neuron death
negative regulation of autophagosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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