Proteintech

CHMP4B Polyclonal Antibody

CHMP4B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

CHMP4B Antibody (13683-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed NIH/3T3 cells using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CHMP4B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

13683-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 25 publications 25 publications

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHMP4B Fusion Protein Ag4544 (1-224 aa encoded by BC033859)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C).

Immunogen sequence: MSVFGKLFG AGGGKAGKGG PTPQEAIQRL RDTEEMLSKK QEFLEKKIEQ ELTAAKKHGT KNKRAALQAL KRKKRYEKQL AQIDGTLSTI EFQREALENA NTNTEVLKNM GYAAKAMKAA HDNMDIDKVD ELMQDIADQQ ELAEEISTAI SKPVGFGEEF DEDELMAELE ELEQEELDKN LLEISGPETV PLPNVPSIAL PSKPAKKKEE EDDDMKELEN WAGSM (1-224 aa encoded by BC033859)

Target Information

CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 UTC 2023

ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.

Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 UTC 2023

ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.

Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 UTC 2023

Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis.

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Jun YW,Lee S,Ban BK,Lee JA,Gao FB

Sat Jul 01 00:00:00 UTC 2023

Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis.

Autophagy

Jun YW,Lee S,Ban BK,Lee JA,Gao FB

Sat Jul 01 00:00:00 UTC 2023

Lysosomal Function and Intracellular Position Determine the Malignant Phenotype in Malignant Melanoma.

The Journal of investigative dermatology

Eriksson I,Vainikka L,Wäster P,Öllinger K

Fri Sep 01 00:00:00 UTC 2023

Long noncoding RNA Sh2d3c promotes manganese-induced neuronal apoptosis through the mmu-miR-675-5p/Chmp4b/Bax axis.

Toxicology letters

Lu W,Li D,Wei S,Yang J,Tang C,Ma X,Zou Y

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The Obligate Intracellular Bacterial Pathogen Anaplasma phagocytophilum Exploits Host Cell Multivesicular Body Biogenesis for Proliferation and Dissemination.

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Tue Dec 20 00:00:00 UTC 2022

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Journal of cardiovascular translational research

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Sat Oct 01 00:00:00 UTC 2022

Polymyxin B Reduces Brain Injury in Ischemic Stroke Rat Through a Mechanism Involving Targeting ESCRT-III Machinery and RIPK1/RIPK3/MLKL Pathway.

Journal of cardiovascular translational research

Tian J,Zhang YY,Peng YW,Liu B,Zhang XJ,Hu ZY,Hu CP,Luo XJ,Peng J

Sat Oct 01 00:00:00 UTC 2022

Polymyxin B Reduces Brain Injury in Ischemic Stroke Rat Through a Mechanism Involving Targeting ESCRT-III Machinery and RIPK1/RIPK3/MLKL Pathway.

Journal of cardiovascular translational research

Tian J,Zhang YY,Peng YW,Liu B,Zhang XJ,Hu ZY,Hu CP,Luo XJ,Peng J

Sat Oct 01 00:00:00 UTC 2022

Polymyxin B Reduces Brain Injury in Ischemic Stroke Rat Through a Mechanism Involving Targeting ESCRT-III Machinery and RIPK1/RIPK3/MLKL Pathway.

Journal of cardiovascular translational research

Tian J,Zhang YY,Peng YW,Liu B,Zhang XJ,Hu ZY,Hu CP,Luo XJ,Peng J

Sat Oct 01 00:00:00 UTC 2022

Ca²⁺-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair.

Nature communications

Niekamp P,Scharte F,Sokoya T,Vittadello L,Kim Y,Deng Y,Südhoff E,Hilderink A,Imlau M,Clarke CJ,Hensel M,Burd CG,Holthuis JCM

Wed Apr 06 00:00:00 UTC 2022

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Neuron

Goldsmith J,Ordureau A,Harper JW,Holzbaur ELF

Wed Mar 16 00:00:00 UTC 2022

BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.

Protein & cell

Wang M,Zhao K,Liu M,Wang M,Qiao Z,Yi S,Jiang Y,Kou X,Zhao Y,Yin J,Li T,Wang H,Jiang C,Gao S,Chen J

Mon Aug 01 00:00:00 UTC 2022

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 UTC 2022

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 UTC 2022

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

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Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome.

Proceedings of the National Academy of Sciences of the United States of America

Dong H,Zhao B,Chen J,Liu Z,Li X,Li L,Wen H

Tue Jun 28 00:00:00 UTC 2022

Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome.

Proceedings of the National Academy of Sciences of the United States of America

Dong H,Zhao B,Chen J,Liu Z,Li X,Li L,Wen H

Tue Jun 28 00:00:00 UTC 2022

A function for ataxia telangiectasia and Rad3-related (ATR) kinase in cytokinetic abscission.

iScience

Luessing J,Okowa CC,Brennan E,Voisin M,Lowndes NF

Fri Jul 15 00:00:00 UTC 2022

Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair.

FEBS letters

Yim WW,Yamamoto H,Mizushima N

Fri Apr 01 00:00:00 UTC 2022

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Science advances

Andrade V,Bai J,Gupta-Rossi N,Jimenez AJ,Delevoye C,Lamaze C,Echard A

Fri Apr 15 00:00:00 UTC 2022

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.

Journal of cell science

Parkinson G,Roboti P,Zhang L,Taylor S,Woodman P

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Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

iScience

Kazan JM,Desrochers G,Martin CE,Jeong H,Kharitidi D,Apaja PM,Roldan A,St Denis N,Gingras AC,Lukacs GL,Pause A

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Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

iScience

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Fri Nov 19 00:00:00 UTC 2021

Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.

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Neggers JE,Paolella BR,Asfaw A,Rothberg MV,Skipper TA,Yang A,Kalekar RL,Krill-Burger JM,Dharia NV,Kugener G,Kalfon J,Yuan C,Dumont N,Gonzalez A,Abdusamad M,Li YY,Spurr LF,Wu WW,Durbin AD,Wolpin BM,Piccioni F,Root DE,Boehm JS,Cherniack AD,Tsherniak A,Hong AL,Hahn WC,Stegmaier K,Golub TR,Vazquez F,Aguirre AJ

Tue Jul 13 00:00:00 UTC 2021

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes.

Cell

Kumar S,Javed R,Mudd M,Pallikkuth S,Lidke KA,Jain A,Tangavelou K,Gudmundsson SR,Ye C,Rusten TE,Anonsen JH,Lystad AH,Claude-Taupin A,Simonsen A,Salemi M,Phinney B,Li J,Guo LW,Bradfute SB,Timmins GS,Eskelinen EL,Deretic V

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Merigliano C,Burla R,La Torre M,Del Giudice S,Teo H,Liew CW,Chojnowski A,Goh WI,Olmos Y,Maccaroni K,Giubettini M,Chiolo I,Carlton JG,Raimondo D,Vernì F,Stewart CL,Rhodes D,Wright GD,Burke BE,Saggio I

Sun Aug 01 00:00:00 UTC 2021

A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.

PLoS biology

Zhang W,Yang X,Chen L,Liu YY,Venkatarangan V,Reist L,Hanson P,Xu H,Wang Y,Li M

Thu Jul 01 00:00:00 UTC 2021

Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

Journal of cell science

Iannantuono NVG,Emery G

Tue Jun 15 00:00:00 UTC 2021

Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.

Cell reports

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Arteriosclerosis, thrombosis, and vascular biology

Rasheed A,Robichaud S,Nguyen MA,Geoffrion M,Wyatt H,Cottee ML,Dennison T,Pietrangelo A,Lee R,Lagace TA,Ouimet M,Rayner KJ

Fri May 01 00:00:00 UTC 2020

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 UTC 2020

Human Cytomegalovirus Utilizes Extracellular Vesicles To Enhance Virus Spread.

Journal of virology

Streck NT,Zhao Y,Sundstrom JM,Buchkovich NJ

Thu Jul 30 00:00:00 UTC 2020

Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins.

Cell death & disease

Eriksson I,Wäster P,Öllinger K

Thu May 14 00:00:00 UTC 2020

LRRK2 activation controls the repair of damaged endomembranes in macrophages.

The EMBO journal

Herbst S,Campbell P,Harvey J,Bernard EM,Papayannopoulos V,Wood NW,Morris HR,Gutierrez MG

Tue Sep 15 00:00:00 UTC 2020

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Methods in molecular biology (Clifton, N.J.)

Adar-Levor S,Goliand I,Elbaum M,Elia N

Mon Mar 02 00:00:00 UTC 2020

A charged multivesicular body protein (CHMP4B) is required for lens growth and differentiation.

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Zhou Y,Bennett TM,Shiels A

Tue Apr 14 00:00:00 UTC 2020

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Current biology : CB

Karasmanis EP,Hwang D,Nakos K,Bowen JR,Angelis D,Spiliotis ET

Mon Jul 08 00:00:00 UTC 2019

Vps4A mediates the localization and exosome release of β-catenin to inhibit epithelial-mesenchymal transition in hepatocellular carcinoma.

Cancer letters

Han Q,Lv L,Wei J,Lei X,Lin H,Li G,Cao J,Xie J,Yang W,Wu S,You J,Lu J,Liu P,Min J

Sat Aug 10 00:00:00 UTC 2019

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

Nonenvelopment Role for the ESCRT-III Complex during Human Cytomegalovirus Infection.

Journal of virology

Streck NT,Carmichael J,Buchkovich NJ

Fri Jun 15 00:00:00 UTC 2018

Nonenvelopment Role for the ESCRT-III Complex during Human Cytomegalovirus Infection.

Journal of virology

Streck NT,Carmichael J,Buchkovich NJ

Fri Jun 15 00:00:00 UTC 2018

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Cell death & disease

Gao K,Zhang Y,Shi Q,Zhang J,Zhang L,Sun H,Jiao D,Zhao X,Tao H,Wei Y,Wang Y,Saiyin H,Zhao SM,Li Y,Zhang P,Wang C

Tue May 01 00:00:00 UTC 2018

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Cell death & disease

Gao K,Zhang Y,Shi Q,Zhang J,Zhang L,Sun H,Jiao D,Zhao X,Tao H,Wei Y,Wang Y,Saiyin H,Zhao SM,Li Y,Zhang P,Wang C

Tue May 01 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 UTC 2018

Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells.

The Journal of cell biology

Kim SB,Kim HR,Park MC,Cho S,Goughnour PC,Han D,Yoon I,Kim Y,Kang T,Song E,Kim P,Choi H,Mun JY,Song C,Lee S,Jung HS,Kim S

Mon Jul 03 00:00:00 UTC 2017

Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells.

The Journal of cell biology

Kim SB,Kim HR,Park MC,Cho S,Goughnour PC,Han D,Yoon I,Kim Y,Kang T,Song E,Kim P,Choi H,Mun JY,Song C,Lee S,Jung HS,Kim S

Mon Jul 03 00:00:00 UTC 2017

ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors.

Science signaling

Mamińska A,Bartosik A,Banach-Orłowska M,Pilecka I,Jastrzębski K,Zdżalik-Bielecka D,Castanon I,Poulain M,Neyen C,Wolińska-Nizioł L,Toruń A,Szymańska E,Kowalczyk A,Piwocka K,Simonsen A,Stenmark H,Fürthauer M,González-Gaitán M,Miaczynska M

Tue Jan 19 00:00:00 UTC 2016

ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors.

Science signaling

Tue Jan 19 00:00:00 UTC 2016

ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.

Journal of cell science

Flores-Rodriguez N,Kenwright DA,Chung PH,Harrison AW,Stefani F,Waigh TA,Allan VJ,Woodman PG

Sun Feb 15 00:00:00 UTC 2015

ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.

Journal of cell science

Flores-Rodriguez N,Kenwright DA,Chung PH,Harrison AW,Stefani F,Waigh TA,Allan VJ,Woodman PG

Sun Feb 15 00:00:00 UTC 2015

Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.

Current biology : CB

Ali N,Zhang L,Taylor S,Mironov A,Urbé S,Woodman P

Mon Mar 18 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Charged multivesicular body protein 4b; CHMP4b; chromatin modifying protein 4B; Chromatin-modifying protein 4b; hSnf7-2; hVps32-2; SNF7 homolog associated with Alix 1; Snf7 homologue associated with Alix 1; SNF7-2; Vacuolar protein sorting-associated protein 32-2; vacuolar protein-sorting-associated protein 32-2; Vps32-2

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Gene Aliases: 2010012F05Rik; C20orf178; C76846; CHMP4A; CHMP4B; CTPP3; CTRCT31; dJ553F4.4; RGD1309846; RGD1565889; SHAX1; SNF7; SNF7-2; Vps32-2; VPS32B

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UniProt ID: (Human) Q9H444, (Mouse) Q9D8B3

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Entrez Gene ID: (Human) 128866, (Mouse) 75608, (Rat) 100359642

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Function(s)

protein binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion

Process(es)

cell separation after cytokinesis posttranslational protein targeting to membrane transport nucleus organization vacuolar transport mitotic metaphase plate congression regulation of centrosome duplication protein transport maintenance of lens transparency viral budding via host ESCRT complex viral budding regulation of viral process protein homooligomerization negative regulation of cell death ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway negative regulation of neuron death regulation of mitotic spindle assembly positive regulation of viral release from host cell negative regulation of autophagosome assembly mitotic cytokinesis autophagy exit from mitosis endosomal transport viral life cycle nuclear envelope reassembly multivesicular body assembly membrane fission cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CHMP4B Polyclonal Antibody

CHMP4B Antibody (13683-1-AP) in ICC/IF

Product Details

13683-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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