Invitrogen

CHOP Polyclonal Antibody

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4 Published Figures
2 References
View all (59) CHOP antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-28956

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human GADD153 Recombinant protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546432

Product Specific Information

Recommended positive controls: HeLa, HeLa treated tunicaycin 8 hr.

Predicted reactivity: Mouse (88%), Rat (90%), Pig (95%), Rhesus Monkey (98%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; Growth arrest and DNA damage-inducible protein GADD153; MGC4154

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Gene Aliases: CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153

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UniProt ID: (Human) P35638

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Entrez Gene ID: (Human) 1649

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding cAMP response element binding protein binding protein homodimerization activity leucine zipper domain binding transcription regulatory region DNA binding protein heterodimerization activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter blood vessel maturation response to amphetamine regulation of transcription, DNA-templated cellular response to DNA damage stimulus ER overload response response to unfolded protein cell cycle arrest aging response to nutrient Wnt signaling pathway positive regulation of interleukin-8 production negative regulation of CREB transcription factor activity response to endoplasmic reticulum stress PERK-mediated unfolded protein response ATF6-mediated unfolded protein response response to drug response to hydrogen peroxide response to starvation mRNA transcription from RNA polymerase II promoter proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process regulation of DNA-templated transcription in response to stress regulation of transcription involved in anterior/posterior axis specification cell redox homeostasis negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic organ development release of sequestered calcium ion into cytosol negative regulation of protein kinase B signaling intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of canonical Wnt signaling pathway positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress negative regulation of determination of dorsal identity
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