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CHRM1 Antibody (711098) in ICC/IF
For immunofluorescence analysis, IMR-32 cells were fixed and permeabilized for detection of endogenous Muscarinic acetylcholine receptor M1 using Anti- Muscarinic acetylcholine receptor M1 Recombinant Rabbit Superclonal™ Antibody (Product # 711098, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Muscarinic acetylcholine receptor M1 protein (green), Panel... View More
Product Details
711098
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
14HCLC
Immunogen
Peptides corresponding to Human CHRM1 (aa 436-450, 327-342)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633122
Product Specific Information
This antibody is predicted to react with Monkey, Pig, House and Rat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor, muscarinic 1; cholinergic receptor; cholinergic receptor, muscarinic 1; M1 mAChR; MGC30125; Muscarinic acetylcholine receptor M1
Gene Aliases: CHRM1; HM1; M1; M1R
UniProt ID: (Human) P11229
Entrez Gene ID: (Human) 1128
regulation of vascular smooth muscle contraction
cellular protein modification process
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
protein kinase C-activating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway
G-protein coupled acetylcholine receptor signaling pathway
synaptic transmission, cholinergic
neuromuscular synaptic transmission
nervous system development
cell proliferation
positive regulation of cell proliferation
regulation of locomotion
positive regulation of ion transport
saliva secretion
cognition
positive regulation of intracellular protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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