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CHRM4 Antibody (AMR-004-200UL) in ICC/IF
Expression of Muscarinic acetylcholine receptorM4in C6 transfected cells - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized C6 cells. A. Transfected cells were stained using Anti-CHRM4 Antibody (#AMR-004), (1:100) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Non-transfected cells were stained using Anti-CHRM4 Antibody (1:100), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). DAPI is used as the counterstain (blue).
Product Details
AMR-004-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor, 3rd intracellular loop
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Muscarinic acetylcholine receptor M4 is an Acetylcholine Receptor. Unlike muscarinic receptor M2, the M4 receptor is not involved in smooth muscle contraction. The clinical relevance of this receptor is unknown. However, mouse studies have linked it to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor, muscarinic 4; AChR M4; cholinergic receptor; cholinergic receptor, muscarin 4; cholinergic receptor, muscarinic 4; Mm4 mAChR; muscarinic acetylcholine receptor 4; Muscarinic acetylcholine receptor M4
Gene Aliases: Chrm-4; CHRM4; HM4; M4; M4R
UniProt ID: (Human) P08173, (Mouse) P32211, (Rat) P08485
Entrez Gene ID: (Human) 1132, (Mouse) 12672, (Rat) 25111
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway
chemical synaptic transmission
synaptic transmission, cholinergic
sensory perception of chemical stimulus
regulation of locomotion
positive regulation of GTPase activity
G-protein coupled acetylcholine receptor signaling pathway
response to organic substance
cell surface receptor signaling pathway
cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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