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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CHRNA1 Antibodies

          Invitrogen

          CHRNA1 Monoclonal Antibody (88B)

          8 References
          View all (9) CHRNA1 antibodies

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          Cite CHRNA1 Monoclonal Antibody (88B)

          • Antibody Testing Data (6)
          CHRNA1 Antibody in Immunocytochemistry (ICC/IF)
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          CHRNA1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          CHRNA1 Antibody (MA3-043) in ICC/IF

          Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product # MA3-043) shows staining in Neuro-2a Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product # MA3-043) at a dilution of 1:100 o... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CHRNA1 Antibody in Immunocytochemistry (ICC/IF)
          CHRNA1 Antibody in Immunocytochemistry (ICC/IF)
          CHRNA1 Antibody in Immunocytochemistry (ICC/IF)
          CHRNA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CHRNA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CHRNA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Product Details

          MA3-043

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000
          View 4 publications 4 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:100
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Fish, Human, Mouse, Rat, Amphibian, Chicken

          Published species

          Human, Mouse, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          88B

          Immunogen

          Purified Torpedo californica acetylcholine receptor.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2081037

          Product Specific Information

          MA3-043 detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit in mouse, human, rat, chicken and frog. This antibody does not detect the alpha 1 and beta 1 subunits.

          MA3-043 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates. Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. Immunohistochemical staining of nAChR in rat skeletal muscle with MA3-043 results in strong staining of the motor endplate.

          In Immunohistochemical staining, cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor.

          The MA3-043 antigen is purified Torpedo californica acetylcholine receptor.

          Target Information

          Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: acetylcholine receptor; Acetylcholine receptor subunit alpha; acetylcholine receptor, nicotinic, alpha 1 (muscle); alpha-1 subunit, nicotinic acetylcholine receptor; cholinergic receptor; cholinergic receptor nicotinic alpha polypeptide 1; cholinergic receptor, nicotinic alpha 1; cholinergic receptor, nicotinic, alpha 1 (muscle); cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle); muscle nicotinic acetylcholine receptor; nAChR; nicotinic acetylcholine receptor alpha subunit; nicotinic cholinergic receptor alpha 1

          View more View less

          Gene Aliases: Achr-1; ACHRA; ACHRD; Acra; AI385656; AI608266; CHNRA; CHRNA; CHRNA1; CMS1A; CMS1B; CMS2A; FCCMS; NACHRA1; SCCMS

          View more View less

          UniProt ID: (Chicken) P09479, (Human) P02708, (Mouse) P04756, (Rat) P25108

          View more View less

          Entrez Gene ID: (Chicken) 395608, (Human) 1134, (Mouse) 11435, (Rat) 79557

          View more View less

          Function(s)
          acetylcholine-activated cation-selective channel activity acetylcholine receptor activity acetylcholine binding ion channel activity ligand-gated ion channel activity extracellular ligand-gated ion channel activity protein binding
          Process(es)
          skeletal muscle contraction neuromuscular synaptic transmission neuromuscular junction development neuronal action potential muscle cell cellular homeostasis skeletal muscle tissue growth neuromuscular process neuron cellular homeostasis transport ion transport cation transport regulation of membrane potential musculoskeletal movement cation transmembrane transport signal transduction synaptic transmission, cholinergic response to nicotine
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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