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CHRNA3 Polyclonal Antibody

CHRNA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CHRNA3 Antibody (10333-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 10333-1-AP) CHRNA3 antibody at dilution of 1:1,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHRNA3 Antibody in Flow Cytometry (Flow)

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Product Details

10333-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHRNA3 Fusion Protein Ag0272 (33-238 aa encoded by BC000513)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EAEHRLFER LFEDYNEIIR PVANVSDPVI IHFEVSMSQL VKVDEVNQIM ETNLWLKQIW NDYKLKWNPS DYGGAEFMRV PAQKIWKPDI VLYNNAVGDF QVDDKTKALL KYTGEVTWIP PAIFKSSCKI DVTYFPFDYQ NCTMKFGSWS YDKAKIDLVL IGSSMNLKDY WESGEWAIIK APGYKHDIKY NCCEEIYPDI TYSLYIR (33-238 aa encoded by BC000513)

Target Information

CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CHRNA5 Is Overexpressed in Patients with Psoriasis and Promotes Psoriasis-Like Inflammation in Mouse Models.

The Journal of investigative dermatology

Wang J,Li X,Zhang P,Yang T,Liu N,Qin L,Ma G,Li X,Fan H,Huang S,Dang N

Tue Nov 01 00:00:00 UTC 2022

A novel murine model of autoimmune dysautonomia by α3 nicotinic acetylcholine receptor immunization.

Frontiers in neuroscience

Yamakawa M,Nakane S,Ihara E,Tawara N,Ikeda H,Igarashi Y,Komohara Y,Takamatsu K,Ikeda T,Tomita Y,Murai S,Ando Y,Mukaino A,Ogawa Y,Ueda M

Wed Dec 21 00:00:00 UTC 2022

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Marine drugs

Sun Z,Zhangsun M,Dong S,Liu Y,Qian J,Zhangsun D,Luo S

Fri Jan 17 00:00:00 UTC 2020

The gut-brain axis rewired: adding a functional vagal nicotinic "sensory synapse".

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Perez-Burgos A,Mao YK,Bienenstock J,Kunze WA

Tue Jul 01 00:00:00 UTC 2014

The gut-brain axis rewired: adding a functional vagal nicotinic "sensory synapse".

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Perez-Burgos A,Mao YK,Bienenstock J,Kunze WA

Tue Jul 01 00:00:00 UTC 2014

Nicotine activates YAP1 through nAChRs mediated signaling in esophageal squamous cell cancer (ESCC).

PloS one

Zhao Y,Zhou W,Xue L,Zhang W,Zhan Q

Fri May 29 00:00:00 UTC 2015

Nicotine activates YAP1 through nAChRs mediated signaling in esophageal squamous cell cancer (ESCC).

PloS one

Zhao Y,Zhou W,Xue L,Zhang W,Zhan Q

Fri May 29 00:00:00 UTC 2015

Nicotine induces fibrogenic changes in human liver via nicotinic acetylcholine receptors expressed on hepatic stellate cells.

Biochemical and biophysical research communications

Soeda J,Morgan M,McKee C,Mouralidarane A,Lin C,Roskams T,Oben JA

Fri Jan 06 00:00:00 UTC 2012

Nicotine induces fibrogenic changes in human liver via nicotinic acetylcholine receptors expressed on hepatic stellate cells.

Biochemical and biophysical research communications

Soeda J,Morgan M,McKee C,Mouralidarane A,Lin C,Roskams T,Oben JA

Fri Jan 06 00:00:00 UTC 2012

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

Human molecular genetics

Strunnikova NV,Maminishkis A,Barb JJ,Wang F,Zhi C,Sergeev Y,Chen W,Edwards AO,Stambolian D,Abecasis G,Swaroop A,Munson PJ,Miller SS

Tue Jun 15 00:00:00 UTC 2010

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

Human molecular genetics

Strunnikova NV,Maminishkis A,Barb JJ,Wang F,Zhi C,Sergeev Y,Chen W,Edwards AO,Stambolian D,Abecasis G,Swaroop A,Munson PJ,Miller SS

Tue Jun 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: acetylcholine receptor; alpha 3; cholinergic receptor; cholinergic receptor, nicotinic alpha 3; cholinergic receptor, nicotinic, alpha 3 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 3; nAChR alpha 3; Neuronal acetylcholine receptor subunit alpha-3; neuronal nicotinic acetylcholine receptor, alpha 3 subunit; neuronal nicotinic acetylcholine receptor, alpha3 subunit

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Gene Aliases: (a)3; A730007P14Rik; Acra-3; Acra3; CHRNA3; LNCR2; NACHRA3; PAOD2

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UniProt ID: (Human) P32297, (Mouse) Q8R4G9

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Entrez Gene ID: (Human) 1136, (Mouse) 110834

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Function(s)

acetylcholine-activated cation-selective channel activity drug binding ligand-gated ion channel activity acetylcholine receptor activity acetylcholine binding protein heterodimerization activity ion channel activity extracellular ligand-gated ion channel activity protein binding

Process(es)

transport ion transport cation transport regulation of muscle contraction regulation of smooth muscle contraction signal transduction activation of transmembrane receptor protein tyrosine kinase activity chemical synaptic transmission synaptic transmission, cholinergic nervous system development locomotory behavior regulation of acetylcholine secretion, neurotransmission behavioral response to nicotine regulation of membrane potential response to drug regulation of dendrite morphogenesis protein heterooligomerization excitatory postsynaptic potential synaptic transmission involved in micturition cation transmembrane transport heart development response to inorganic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CHRNA3 Polyclonal Antibody

CHRNA3 Antibody (10333-1-AP) in IHC (P)

Product Details

10333-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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