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CHX10 Antibody (PA5-95472) in WB
Western blot analysis of CHX10 in Lane 1: mouse eye ball tissue lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with CHX10 polyclonal antibody (Product # PA5-95472) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Product Details
PA5-95472
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CHX10 recombinant protein (Position: A249-A361).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807274
Product Specific Information
Human CHX10 shares 90.3% amino acid (aa) sequence identity with mouse CHX10.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse eye ball tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
CHX10 encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C. elegans ceh-10 homeo domain containing homolog; ceh-10 homeo domain containing homolog; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10; ocular retardation; Visual system homeobox 2
Gene Aliases: CHX10; Hox-10; HOX10; MCOP2; MCOPCB3; or; RET1; VSX2
UniProt ID: (Human) P58304, (Mouse) Q61412
Entrez Gene ID: (Human) 338917, (Mouse) 12677
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
positive regulation of cell proliferation
negative regulation of cell proliferation
camera-type eye development
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
retinal bipolar neuron differentiation
retina morphogenesis in camera-type eye
visual perception
response to stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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