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Invitrogen
CIDEA Polyclonal Antibody
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FIGURE: 1 / 2
CIDEA Antibody (PA5-80477) in ICC/IF
Immunofluorescence staining of CIDEA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CIDEA Polyclonal Antibody (Product # PA5-80477, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-80477
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human CIDEA
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787790
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human CIDEA.
Target Information
This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell death activator CIDE-A; Cell death-inducing DFFA-like effector A; cide a; Lipid transferase CIDEA
Gene Aliases: CIDE-A; CIDEA
UniProt ID: (Human) O60543
Entrez Gene ID: (Human) 1149
temperature homeostasis
transcription, DNA-templated
regulation of transcription, DNA-templated
lipid metabolic process
apoptotic process
cell death
positive regulation of sequestering of triglyceride
lipid storage
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of tumor necrosis factor production
response to stilbenoid
regulation of apoptotic process
negative regulation of cytokine secretion
negative regulation of lipid catabolic process
negative regulation of execution phase of apoptosis
regulation of apoptotic DNA fragmentation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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