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Invitrogen
CIS Polyclonal Antibody
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FIGURE: 1 / 9
CIS Antibody (PA5-79048) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CIS in U2OS cells using CIS Polyclonal Antibody (Product # PA5-79048). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets ap... View More
Product Details
PA5-79048
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CISH (241-258aa LPLPRRMADYLRQYPFQL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746164
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human Hela whole cell, human COLO320 whole cell, human CACO-2 whole cell. IHC: Human Lung Cancer Tissue, Rat Kidney Tissue. ICC/IF: U2OS cells.
Target Information
CIS contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CIS; CIS-1; cytokine inducible SH2-containing protein; cytokine inducible SH2-containing protein 1; cytokine-inducible inhibitor of signaling type 1B; cytokine-inducible SH2 protein; Cytokine-inducible SH2-containing protein; Protein G18; SOCS; Suppressor of cytokine signaling
Gene Aliases: AI385595; BACTS2; CIS; CIS-1; CIS1; CISH; F17; F23; G18; SOCS
UniProt ID: (Human) Q9NSE2, (Mouse) Q62225
Entrez Gene ID: (Human) 1154, (Rat) 83681, (Mouse) 12700
regulation of cell growth
negative regulation of protein kinase activity
protein kinase C-activating G-protein coupled receptor signaling pathway
protein ubiquitination
cytokine-mediated signaling pathway
intracellular signal transduction
negative regulation of JAK-STAT cascade
negative regulation of insulin receptor signaling pathway
regulation of growth
negative regulation of signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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