Proteintech

CISD2 Polyclonal Antibody

CISD2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 26

CISD2 Antibody (13318-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 13318-1-AP (CISD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

CISD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CISD2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13318-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CISD2 Fusion Protein Ag4172 (61-135 aa encoded by BC032300)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PKKKQQKDS LINLKIQKEN PKVVNEINIE DLCLTKAAYC RCWRSKTFPA CDGSHNKHNE LTGDNVGPLI LKKKEV (61-135 aa encoded by BC032300)

Target Information

Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Delivery of low-density lipoprotein from endocytic carriers to mitochondria supports steroidogenesis.

Nature cell biology

Zhou YX,Wei J,Deng G,Hu A,Sun PY,Zhao X,Song BL,Luo J

Sat Jul 01 00:00:00 UTC 2023

Delivery of low-density lipoprotein from endocytic carriers to mitochondria supports steroidogenesis.

Nature cell biology

Zhou YX,Wei J,Deng G,Hu A,Sun PY,Zhao X,Song BL,Luo J

Sat Jul 01 00:00:00 UTC 2023

CDGSH iron sulfur domain 2 over-expression alleviates neuronal ferroptosis and brain injury by inhibiting lipid peroxidation via AKT/mTOR pathway following intracerebral hemorrhage in mice.

Journal of neurochemistry

Li R,Zhang X,Gu L,Yuan Y,Luo X,Shen W,Xie Z

Mon May 01 00:00:00 UTC 2023

CDGSH iron sulfur domain 2 over-expression alleviates neuronal ferroptosis and brain injury by inhibiting lipid peroxidation via AKT/mTOR pathway following intracerebral hemorrhage in mice.

Journal of neurochemistry

Li R,Zhang X,Gu L,Yuan Y,Luo X,Shen W,Xie Z

Mon May 01 00:00:00 UTC 2023

CISD2 promotes lung squamous carcinoma cell migration and invasion via the TGF-β1-induced Smad2/3 signaling pathway.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

Zhang J,Pan L,Zhang S,Yang Y,Liang J,Ma S,Wu Q

Fri Dec 01 00:00:00 UTC 2023

CISD2 promotes lung squamous carcinoma cell migration and invasion via the TGF-β1-induced Smad2/3 signaling pathway.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

Zhang J,Pan L,Zhang S,Yang Y,Liang J,Ma S,Wu Q

Fri Dec 01 00:00:00 UTC 2023

CDGSH iron sulfur domain 2 mitigates apoptosis, oxidative stress and inflammatory response caused by oxygen-glucose deprivation/reoxygenation in HT22 hippocampal neurons by Akt-Nrf2-activated pathway.

Metabolic brain disease

Ren X,Yu J,Guo L,Zhang Z

Sat Oct 01 00:00:00 UTC 2022

CISD2 Promotes Resistance to Sorafenib-Induced Ferroptosis by Regulating Autophagy in Hepatocellular Carcinoma.

Frontiers in oncology

Li B,Wei S,Yang L,Peng X,Ma Y,Wu B,Fan Q,Yang S,Li X,Jin H,Tang S,Huang M,Li H,Liu J

Tue Apr 26 00:00:00 UTC 2022

CISD2 Promotes Resistance to Sorafenib-Induced Ferroptosis by Regulating Autophagy in Hepatocellular Carcinoma.

Frontiers in oncology

Li B,Wei S,Yang L,Peng X,Ma Y,Wu B,Fan Q,Yang S,Li X,Jin H,Tang S,Huang M,Li H,Liu J

Tue Apr 26 00:00:00 UTC 2022

New Insights of the NEET Protein CISD2 Reveals Distinct Features Compared to Its Close Mitochondrial Homolog mitoNEET.

Biomedicines

Salameh M,Riquier S,Guittet O,Huang ME,Vernis L,Lepoivre M,Golinelli-Cohen MP

Mon Apr 05 00:00:00 UTC 2021

Cryogen spray cooling mitigates inflammation and injury-induced CISD2 decline in rat spinal cord hemisection model.

Journal of integrative neuroscience

Kung WM,Chang CJ,Chen TY,Lin MS

Wed Dec 30 00:00:00 UTC 2020

Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.

EMBO reports

Molino D,Pila-Castellanos I,Marjault HB,Dias Amoedo N,Kopp K,Rochin L,Karmi O,Sohn YS,Lines L,Hamaï A,Joly S,Radreau P,Vonderscher J,Codogno P,Giordano F,Machin P,Rossignol R,Meldrum E,Arnoult D,Ruggieri A,Nechushtai R,de Chassey B,Morel E

Thu Dec 03 00:00:00 UTC 2020

NAF-1 Inhibition by Resveratrol Suppresses Cancer Stem Cell-Like Properties and the Invasion of Pancreatic Cancer.

Frontiers in oncology

Qin T,Cheng L,Xiao Y,Qian W,Li J,Wu Z,Wang Z,Xu Q,Duan W,Wong L,Wu E,Ma Q,Ma J

Mon Sep 28 00:00:00 UTC 2020

NAF-1 Inhibition by Resveratrol Suppresses Cancer Stem Cell-Like Properties and the Invasion of Pancreatic Cancer.

Frontiers in oncology

Qin T,Cheng L,Xiao Y,Qian W,Li J,Wu Z,Wang Z,Xu Q,Duan W,Wong L,Wu E,Ma Q,Ma J

Mon Sep 28 00:00:00 UTC 2020

NAF-1 Inhibition by Resveratrol Suppresses Cancer Stem Cell-Like Properties and the Invasion of Pancreatic Cancer.

Frontiers in oncology

Qin T,Cheng L,Xiao Y,Qian W,Li J,Wu Z,Wang Z,Xu Q,Duan W,Wong L,Wu E,Ma Q,Ma J

Mon Sep 28 00:00:00 UTC 2020

A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Curley M,Milne L,Smith S,Jørgensen A,Frederiksen H,Hadoke P,Potter P,Smith LB

Tue Jan 01 00:00:00 UTC 2019

A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Curley M,Milne L,Smith S,Jørgensen A,Frederiksen H,Hadoke P,Potter P,Smith LB

Tue Jan 01 00:00:00 UTC 2019

A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Curley M,Milne L,Smith S,Jørgensen A,Frederiksen H,Hadoke P,Potter P,Smith LB

Tue Jan 01 00:00:00 UTC 2019

A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Curley M,Milne L,Smith S,Jørgensen A,Frederiksen H,Hadoke P,Potter P,Smith LB

Tue Jan 01 00:00:00 UTC 2019

Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways.

Oxidative medicine and cellular longevity

Cheng L,Yan B,Chen K,Jiang Z,Zhou C,Cao J,Qian W,Li J,Sun L,Ma J,Ma Q,Sha H

Fri Sep 21 00:00:00 UTC 2018

Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways.

Oxidative medicine and cellular longevity

Cheng L,Yan B,Chen K,Jiang Z,Zhou C,Cao J,Qian W,Li J,Sun L,Ma J,Ma Q,Sha H

Fri Sep 21 00:00:00 UTC 2018

Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways.

Oxidative medicine and cellular longevity

Cheng L,Yan B,Chen K,Jiang Z,Zhou C,Cao J,Qian W,Li J,Sun L,Ma J,Ma Q,Sha H

Fri Sep 21 00:00:00 UTC 2018

Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways.

Oxidative medicine and cellular longevity

Cheng L,Yan B,Chen K,Jiang Z,Zhou C,Cao J,Qian W,Li J,Sun L,Ma J,Ma Q,Sha H

Fri Sep 21 00:00:00 UTC 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 UTC 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 UTC 2018

CISD2 enhances the chemosensitivity of gastric cancer through the enhancement of 5-FU-induced apoptosis and the inhibition of autophagy by AKT/mTOR pathway.

Cancer medicine

Sun Y,Jiang Y,Huang J,Chen H,Liao Y,Yang Z

Sun Oct 01 00:00:00 UTC 2017

CISD2 enhances the chemosensitivity of gastric cancer through the enhancement of 5-FU-induced apoptosis and the inhibition of autophagy by AKT/mTOR pathway.

Cancer medicine

Sun Y,Jiang Y,Huang J,Chen H,Liao Y,Yang Z

Sun Oct 01 00:00:00 UTC 2017

CISD2 enhances the chemosensitivity of gastric cancer through the enhancement of 5-FU-induced apoptosis and the inhibition of autophagy by AKT/mTOR pathway.

Cancer medicine

Sun Y,Jiang Y,Huang J,Chen H,Liao Y,Yang Z

Sun Oct 01 00:00:00 UTC 2017

CISD2 enhances the chemosensitivity of gastric cancer through the enhancement of 5-FU-induced apoptosis and the inhibition of autophagy by AKT/mTOR pathway.

Cancer medicine

Sun Y,Jiang Y,Huang J,Chen H,Liao Y,Yang Z

Sun Oct 01 00:00:00 UTC 2017

A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.

BMC medical genetics

Cattaneo M,La Sala L,Rondinelli M,Errichiello E,Zuffardi O,Puca AA,Genovese S,Ceriello A

Wed Dec 13 00:00:00 UTC 2017

A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.

BMC medical genetics

Cattaneo M,La Sala L,Rondinelli M,Errichiello E,Zuffardi O,Puca AA,Genovese S,Ceriello A

Wed Dec 13 00:00:00 UTC 2017

Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

Oncotarget

Wang L,Ouyang F,Liu X,Wu S,Wu HM,Xu Y,Wang B,Zhu J,Xu X,Zhang L

Tue Jan 26 00:00:00 UTC 2016

Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

Oncotarget

Wang L,Ouyang F,Liu X,Wu S,Wu HM,Xu Y,Wang B,Zhu J,Xu X,Zhang L

Tue Jan 26 00:00:00 UTC 2016

Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

Oncotarget

Wang L,Ouyang F,Liu X,Wu S,Wu HM,Xu Y,Wang B,Zhu J,Xu X,Zhang L

Tue Jan 26 00:00:00 UTC 2016

Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

Oncotarget

Wang L,Ouyang F,Liu X,Wu S,Wu HM,Xu Y,Wang B,Zhu J,Xu X,Zhang L

Tue Jan 26 00:00:00 UTC 2016

A novel prognostic score model incorporating CDGSH iron sulfur domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

Oncotarget

Yang L,Hong S,Wang Y,He Z,Liang S,Chen H,He S,Wu S,Song L,Chen Y

Tue Apr 19 00:00:00 UTC 2016

A novel prognostic score model incorporating CDGSH iron sulfur domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

Oncotarget

Yang L,Hong S,Wang Y,He Z,Liang S,Chen H,He S,Wu S,Song L,Chen Y

Tue Apr 19 00:00:00 UTC 2016

A novel prognostic score model incorporating CDGSH iron sulfur domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

Oncotarget

Yang L,Hong S,Wang Y,He Z,Liang S,Chen H,He S,Wu S,Song L,Chen Y

Tue Apr 19 00:00:00 UTC 2016

A novel prognostic score model incorporating CDGSH iron sulfur domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

Oncotarget

Yang L,Hong S,Wang Y,He Z,Liang S,Chen H,He S,Wu S,Song L,Chen Y

Tue Apr 19 00:00:00 UTC 2016

CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

International journal of clinical and experimental pathology

Chen B,Shen S,Wu J,Hua Y,Kuang M,Li S,Peng B

Tue Dec 13 00:00:00 UTC 2016

CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

International journal of clinical and experimental pathology

Chen B,Shen S,Wu J,Hua Y,Kuang M,Li S,Peng B

Tue Dec 13 00:00:00 UTC 2016

CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

International journal of clinical and experimental pathology

Chen B,Shen S,Wu J,Hua Y,Kuang M,Li S,Peng B

Tue Dec 13 00:00:00 UTC 2016

CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

International journal of clinical and experimental pathology

Chen B,Shen S,Wu J,Hua Y,Kuang M,Li S,Peng B

Tue Dec 13 00:00:00 UTC 2016

Sampling the conformational space of the catalytic subunit of human γ-secretase.

eLife

Bai XC,Rajendra E,Yang G,Shi Y,Scheres SH

Tue Dec 01 00:00:00 UTC 2015

A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.

Proceedings of the National Academy of Sciences of the United States of America

Lu S,Kanekura K,Hara T,Mahadevan J,Spears LD,Oslowski CM,Martinez R,Yamazaki-Inoue M,Toyoda M,Neilson A,Blanner P,Brown CM,Semenkovich CF,Marshall BA,Hershey T,Umezawa A,Greer PA,Urano F

Tue Dec 09 00:00:00 UTC 2014

A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.

Proceedings of the National Academy of Sciences of the United States of America

Lu S,Kanekura K,Hara T,Mahadevan J,Spears LD,Oslowski CM,Martinez R,Yamazaki-Inoue M,Toyoda M,Neilson A,Blanner P,Brown CM,Semenkovich CF,Marshall BA,Hershey T,Umezawa A,Greer PA,Urano F

Tue Dec 09 00:00:00 UTC 2014

A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.

Proceedings of the National Academy of Sciences of the United States of America

Lu S,Kanekura K,Hara T,Mahadevan J,Spears LD,Oslowski CM,Martinez R,Yamazaki-Inoue M,Toyoda M,Neilson A,Blanner P,Brown CM,Semenkovich CF,Marshall BA,Hershey T,Umezawa A,Greer PA,Urano F

Tue Dec 09 00:00:00 UTC 2014

A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.

Proceedings of the National Academy of Sciences of the United States of America

Lu S,Kanekura K,Hara T,Mahadevan J,Spears LD,Oslowski CM,Martinez R,Yamazaki-Inoue M,Toyoda M,Neilson A,Blanner P,Brown CM,Semenkovich CF,Marshall BA,Hershey T,Umezawa A,Greer PA,Urano F

Tue Dec 09 00:00:00 UTC 2014

CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer.

Medical oncology (Northwood, London, England)

Liu L,Xia M,Wang J,Zhang W,Zhang Y,He M

Mon Sep 01 00:00:00 UTC 2014

CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer.

Medical oncology (Northwood, London, England)

Liu L,Xia M,Wang J,Zhang W,Zhang Y,He M

Mon Sep 01 00:00:00 UTC 2014

CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer.

Medical oncology (Northwood, London, England)

Liu L,Xia M,Wang J,Zhang W,Zhang Y,He M

Mon Sep 01 00:00:00 UTC 2014

CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer.

Medical oncology (Northwood, London, England)

Liu L,Xia M,Wang J,Zhang W,Zhang Y,He M

Mon Sep 01 00:00:00 UTC 2014

Genetic studies in Drosophila and humans support a model for the concerted function of CISD2, PPT1 and CLN3 in disease.

Biology open

Jones MA,Amr S,Ferebee A,Huynh P,Rosenfeld JA,Miles MF,Davies AG,Korey CA,Warrick JM,Shiang R,Elsea SH,Girirajan S,Grotewiel M

Fri Apr 04 00:00:00 UTC 2014

Genetic studies in Drosophila and humans support a model for the concerted function of CISD2, PPT1 and CLN3 in disease.

Biology open

Jones MA,Amr S,Ferebee A,Huynh P,Rosenfeld JA,Miles MF,Davies AG,Korey CA,Warrick JM,Shiang R,Elsea SH,Girirajan S,Grotewiel M

Fri Apr 04 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CDGSH iron sulfur domain-containing protein 2; CDGSH iron-sulfur domain-containing protein 2; Endoplasmic reticulum intermembrane small protein; Miner1; mitoNEET related 1; MitoNEET-related 1 protein; NAF-1; nervous system over-expressed protein 70; Nervous system overexpressed protein 70; Nutrient-deprivation autophagy factor-1; zinc finger, CDGSH-type domain 2

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Gene Aliases: 1500009M05Rik; 1500026J14Rik; 1500031D15Rik; AI848398; B630006A20Rik; CDGSH2; CISD2; ERIS; Miner1; NAF-1; Noxp70; RGD1566242; WFS2; ZCD2

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UniProt ID: (Human) Q8N5K1, (Mouse) Q9CQB5

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Entrez Gene ID: (Human) 493856, (Rat) 295457, (Mouse) 67006

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Function(s)

protein binding protein homodimerization activity poly(A) RNA binding metal ion binding 2 iron, 2 sulfur cluster binding iron-sulfur cluster binding

Process(es)

mitophagy multicellular organism aging regulation of autophagy autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CISD2 Polyclonal Antibody

CISD2 Antibody (13318-1-AP) in ICC/IF

Product Details

13318-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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