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FIGURE: 1 / 3
CKAP1 Antibody (PA5-118302) in ICC/IF
Immunofluorescence staining of CKAP1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with CKAP1 Polyclonal Antibody (Product # PA5-118302, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and cell membrane.
Product Details
PA5-118302
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human CKAP1/TBCB fragment
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2902897
Target Information
CKAP1 binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoskeleton associated protein 1; Cytoskeleton-associated protein 1; Cytoskeleton-associated protein CKAPI; Tubulin-folding cofactor B; Tubulin-specific chaperone B
Gene Aliases: CG22; CKAP1; CKAPI; TBCB
UniProt ID: (Human) Q99426
Entrez Gene ID: (Human) 1155
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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