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Invitrogen
CLCN3 Monoclonal Antibody (N258/5), PE
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FIGURE: 1 / 3
CLCN3 Antibody (MA5-45557) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Clcn3 monoclonal antibody (Product # MA5-45557) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification:... View More
Product Details
MA5-45557
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N258/5
Immunogen
Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932011
Product Specific Information
1 µg/mL of MA5-45557 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
This antibody was formerly sold as clone S258-5.
Target Information
Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chloride channel 3; Chloride channel protein 3; chloride channel, voltage-sensitive 3; Chloride transporter ClC-3; CIC3; ClC-3; CLCN 3; DKFZp564I0463; H(+)/Cl(-) exchange transporter 3; protein kinase C-regulated chloride channel
Gene Aliases: ClC-3; CLC3; CLCN3
UniProt ID: (Human) P51790, (Rat) P51792, (Mouse) P51791
Entrez Gene ID: (Human) 1182, (Rat) 84360, (Mouse) 12725
voltage-gated chloride channel activity
chloride channel activity
protein binding
ATP binding
antiporter activity
PDZ domain binding
chloride ion binding
protein homodimerization activity
protein heterodimerization activity
volume-sensitive chloride channel activity
ion channel activity
voltage-gated ion channel activity
nucleotide binding
ion transport
chloride transport
phagocytosis, engulfment
adult locomotory behavior
ion transmembrane transport
regulation of ion transmembrane transport
synaptic transmission, glutamatergic
regulation of anion transport
photoreceptor cell maintenance
negative regulation of cell volume
synaptic transmission, GABAergic
neuron cellular homeostasis
synaptic vesicle lumen acidification
chloride transmembrane transport
positive regulation of reactive oxygen species biosynthetic process
transport
regulation of pH
endosomal lumen acidification
regulation of anion transmembrane transport
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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