Invitrogen

CLOCK Polyclonal Antibody

CLOCK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

CLOCK Antibody (PA1-520) in ICC/IF

Immunofluorescent analysis of CLOCK (green) showing staining in the nucleus and cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CLOCK polyclonal antibody (Product # PA1-520) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More

CLOCK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CLOCK Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CLOCK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. SATB1 was observed in Mouse Skeletal Muscle but not in Mouse Liver using CLOCK Polyclonal Antibody (Product # PA1-520) in western blot. {RE}

Product Details

PA1-520

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

View 2 publications 2 publications

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: S(11) S I V D R D D S S I F D G L V E E D D(30) C

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2082586

Product Specific Information

PA1-520 detects CLOCK from human, mouse, rat and hamster tissues.

PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus.

PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. # PEP-072) is available for use in neutralization and control experiments.

Target Information

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations.

PloS one

Vyas H,Vohra A,Upadhyay K,Thounaojam M,Jadeja R,Dalvi N,Bartoli M,Devkar R

Published figure using CLOCK polyclonal antibody (Product # PA1-520) in Western Blot

Tue Aug 15 00:00:00 UTC 2023

miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations.

PloS one

Vyas H,Vohra A,Upadhyay K,Thounaojam M,Jadeja R,Dalvi N,Bartoli M,Devkar R

Published figure using CLOCK polyclonal antibody (Product # PA1-520) in Western Blot

Tue Aug 15 00:00:00 UTC 2023

Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model.

Cellular and molecular gastroenterology and hepatology

Ceci L,Chen L,Baiocchi L,Wu N,Kennedy L,Carpino G,Kyritsi K,Zhou T,Owen T,Kundu D,Sybenga A,Isidan A,Ekser B,Franchitto A,Onori P,Gaudio E,Mancinelli R,Francis H,Alpini G,Glaser S

Published figure using CLOCK polyclonal antibody (Product # PA1-520) in Immunocytochemistry

Mon Sep 26 00:00:00 UTC 2022

Melatonin induces Nrf2-HO-1 reprogramming and corrections in hepatic core clock oscillations in Non-alcoholic fatty liver disease.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Joshi A,Upadhyay KK,Vohra A,Shirsath K,Devkar R

PA1-520 was used in Western Blot to investigate perturbations in the hepatic circadian clock gene, and Nrf2-HO-1 oscillations in conditions of high-fat high fructose (HFHF) diet and/or jet lag (JL)-mediated NAFLD.

Wed Sep 01 00:00:00 UTC 2021

Circadian Oscillations Persist in Cervical and Esophageal Cancer Cells Displaying Decreased Expression of Tumor-Suppressing Circadian Clock Genes.

Molecular cancer research : MCR

van der Watt PJ,Roden LC,Davis KT,Parker MI,Leaner VD

PA1-520 was used in Western Blot to describe dysregulated, yet oscillating, circadian clock gene expression in cervical and esophageal cancer cells, and knowledge of circadian clock functioning in these cancer types has the potential to inform chronotherapy approaches, where the timing of administration of chemotherapy is optimized on the basis of the circadian clock.

Tue Sep 01 00:00:00 UTC 2020

Melatonin modulates daytime-dependent synaptic plasticity and learning efficiency.

Journal of pineal research

Jilg A,Bechstein P,Saade A,Dick M,Li TX,Tosini G,Rami A,Zemmar A,Stehle JH

PA1-520 was used in Immunohistochemistry to investigate the role of the neurohormone melatonin as a circadian time cue for hippocampal signaling and memory formation.

Mon Apr 01 00:00:00 UTC 2019

Circadian networks in human embryonic stem cell-derived cardiomyocytes.

EMBO reports

Dierickx P,Vermunt MW,Muraro MJ,Creyghton MP,Doevendans PA,van Oudenaarden A,Geijsen N,Van Laake LW

Published figure using CLOCK polyclonal antibody (Product # PA1-520) in Western Blot

Sat Jul 01 00:00:00 UTC 2017

Circadian networks in human embryonic stem cell-derived cardiomyocytes.

EMBO reports

Dierickx P,Vermunt MW,Muraro MJ,Creyghton MP,Doevendans PA,van Oudenaarden A,Geijsen N,Van Laake LW

Published figure using CLOCK polyclonal antibody (Product # PA1-520) in Western Blot

Sat Jul 01 00:00:00 UTC 2017

Time-caloric restriction inhibits the neoplastic transformation of cirrhotic liver in rats treated with diethylnitrosamine.

Carcinogenesis

Molina-Aguilar C,Guerrero-Carrillo MJ,Espinosa-Aguirre JJ,Olguin-Reyes S,Castro-Belio T,Vázquez-Martínez O,Rivera-Zavala JB,Díaz-Muñoz M

PA1-520 was used in Western Blotting to conclude that time-caloric restriction prevents fibrosis from progressing into cirrhosis, thus avoiding chronic inflammation and regenerative processes.

Tue Aug 01 00:00:00 UTC 2017

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.

PloS one

Boström J,Sramkova Z,Salašová A,Johard H,Mahdessian D,Fedr R,Marks C,Medalová J,Souček K,Lundberg E,Linnarsson S,Bryja V,Sekyrova P,Altun M,Andäng M

PA1-520 was used in Immunocytochemistry-immunoflourescence to characterise the transcriptomes of unsynchronised HeLa and U2OS cancer cells, sorted for cell cycle phase by Fucci reporter expression.

Thu Jan 04 00:00:00 UTC 2018

Diurnal oscillation of amygdala clock gene expression and loss of synchrony in a mouse model of depression.

The international journal of neuropsychopharmacology

Savalli G,Diao W,Schulz S,Todtova K,Pollak DD

PA1-520 was used in immunohistochemistry - free floating to discuss the contribution of the circadian system to depression.

Thu Dec 11 00:00:00 UTC 2014

Circadian rhythms regulate amelogenesis.

Bone

Zheng L,Seon YJ,Mourão MA,Schnell S,Kim D,Harada H,Papagerakis S,Papagerakis P

PA1-520 was used in western blot to study the circadian regulation of amelogenesis

Mon Jul 01 00:00:00 UTC 2013

p53 regulates Period2 expression and the circadian clock.

Nature communications

Miki T,Matsumoto T,Zhao Z,Lee CC

PA1-520 was used in EMSA to assess the role of p53 in regulating Period2 expression and the circadian clock

Mon Apr 21 00:00:00 UTC 2014

Expression of clock proteins in developing tooth.

Gene expression patterns : GEP

Zheng L,Papagerakis S,Schnell SD,Hoogerwerf WA,Papagerakis P

PA1-520 was used in immunohistochemistry to study the role of clock genes in developing tooth

Thu Jul 21 00:00:00 UTC 2011

The E2F-regulated gene Chk1 is highly expressed in triple-negative estrogen receptor /progesterone receptor /HER-2 breast carcinomas.

Cancer research

Verlinden L,Vanden Bempt I,Eelen G,Drijkoningen M,Verlinden I,Marchal K,De Wolf-Peeters C,Christiaens MR,Michiels L,Bouillon R,Verstuyf A

PA1-520 was used in immunohistochemistry to study the expression of Chk1 in triple-negative estrogen receptor/progesterone receptor/HER-2 breast carcinomas

Sun Jul 15 00:00:00 UTC 2007

Non-cyclic and developmental stage-specific expression of circadian clock proteins during murine spermatogenesis.

Biology of reproduction

Alvarez JD,Chen D,Storer E,Sehgal A

PA1-520 was used in immunohistochemistry to investigate the expression of circadian clock proteins during murine spermatogenesis

Tue Jul 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: bHLHe8; circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock circadian regulator; clock homolog; hCLOCK; mCLOCK; rCLOCK

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Gene Aliases: 5330400M04Rik; BHLHE8; CLOCK; I79_020252; KAT13D; KIAA0334

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UniProt ID: (Human) O15516, (Mouse) O08785, (Rat) Q9WVS9

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Entrez Gene ID: (Human) 9575, (Chinese hamster) 100770092, (Mouse) 12753, (Rat) 60447

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding histone acetyltransferase activity protein binding chromatin DNA binding sequence-specific DNA binding protein dimerization activity E-box binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding signal transducer activity transcription factor binding transferase activity transferase activity, transferring acyl groups

Process(es)

DNA damage checkpoint transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cellular response to DNA damage stimulus signal transduction spermatogenesis circadian rhythm histone acetylation circadian regulation of gene expression regulation of hair cycle proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of inflammatory response regulation of insulin secretion positive regulation of NF-kappaB transcription factor activity response to redox state cellular response to ionizing radiation regulation of type B pancreatic cell development negative regulation of glucocorticoid receptor signaling pathway regulation of transcription from RNA polymerase II promoter photoperiodism entrainment of circadian clock

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CLOCK Polyclonal Antibody

CLOCK Antibody (PA1-520) in ICC/IF

Product Details

PA1-520

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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