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Invitrogen
CLPX Monoclonal Antibody (11C11)
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FIGURE: 1 / 3
CLPX Antibody (MA5-36237) in ICC/IF
Immunocytochemistry analysis of CLPX in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in CLPX monoclonal antibody (Product # MA5-36237) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
11C11
Immunogen
E. coli-derived human CLPX recombinant protein (Position: A337-E574).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884071
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji tissue, human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell. ICC/IF: MCF7 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; ATP-dependent clpX-like chaperone, mitochondrial; Caseinolytic mitochondrial matrix peptidase chaperone subunit X; ClpX caseinolytic peptidase X homolog; ClpX caseinolytic protease X homolog; energy-dependent regulator of proteolysis
Gene Aliases: CLPX
UniProt ID: (Human) O76031
Entrez Gene ID: (Human) 10845
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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