Invitrogen

CLUH Polyclonal Antibody

FIGURE: 1 / 2

CLUH Antibody (PA5-71324) in WB

Western blot analysis of CLUH on human MDA-MB-435s cells. The sample was probed with a CLUH polyclonal antibody (Product # PA5-71324) using a primary antibody dilution of 1.0 µg/mL.

Product Image Gallery

CLUH Antibody — Figure 8. Knockdown of the clustered mitochondria homolog (CLUH) elicits perinuclear mitochondrial redistribution, elevated mitochondrial ROS generation, and enhances RSV virus production. ( A-E ) A549 cells were pretreated (48 hr) with siRNA (50 nM) for clustered mitochondria homolog ( CLUH ), or scrambled siRNA control (scr) followed by infection with RSV (MOI 1) for another 24 hr. ( A ) Immunoblot analysis for CLUH , or the control alpha-tubulin, as indicated (40 ug cell lysate protein/lane). ( B and C ) Immunostaining and R 90% analysis were as per ( Figure 1C and D ). In all panels, scale bar = 10 um. Results represent the mean +- SEM for n = 3 independent experiments, where each experiment analysed 25-30 cells per sample; ***p<0.001. ( D ) Cells were stained with Mitotracker Deep Red and the mitochondria-specific ROS probe FRR2 as per Figure 7A . The ratiometric output images of I (Ex514) /I (Ex488) were calculated as per Figure 7A . ( E ) FRR2 (I (Ex514) /I (Ex488) ) was calculated for the mitochondrial regions defined by Mitotracker Deep Red staining in the I (Ex514) /I (Ex488) images such as those in D ) using a custom CellProfiler pipeline as per Figure 7B . Results represent the mean +- SEM for n = 3 independent experiments, where each experiment analysed 25-30 cells per sample, ***p<0.001, **p<0.01. Figure 8--figure supplement 1. Microtubule/dynein-dependent mitochondrial redistribution favours RSV virus production. Infectious virus (plaque forming units pfu ml -1 {KD}

Product Details

PA5-71324

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide directed towards the C-terminal region of human CLUH

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2690757

Target Information

mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.

eLife

Hu M,Schulze KE,Ghildyal R,Henstridge DC,Kolanowski JL,New EJ,Hong Y,Hsu AC,Hansbro PM,Wark PA,Bogoyevitch MA,Jans DA

PA5-71324 was used in Western Blot to establish respiratory syncytial viruses unique ability to co-opt host cell mitochondria to facilitate viral infection, revealing the RSV-mitochondrial interface for the first time as a viable target for therapeutic intervention.

Thu Jun 27 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: clustered mitochondria (cluA/CLU1) homolog; Clustered mitochondria protein homolog

View more View less

Gene Aliases: CLU1; CLUH; KIAA0664

View more View less

UniProt ID: (Human) O75153

View more View less

Entrez Gene ID: (Human) 23277

View more View less

Function(s)

mRNA binding

Process(es)

mitochondrion organization intracellular distribution of mitochondria

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CLUH Polyclonal Antibody

CLUH Antibody (PA5-71324) in WB

Product Details

PA5-71324

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!