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Invitrogen
CNOT1 Polyclonal Antibody
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CNOT1 Antibody (PA5-99966) in IHC (P)
Immunohistochemistry analysis of CNOT1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CNOT1 polyclonal antibody (Product # PA5-99966) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99966
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CNOT1(Accession A5YKK6), corresponding to amino acid residues S785-E835.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818899
Product Specific Information
Antibody detects endogenous levels of total CNOT1.
Target Information
Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenal gland protein AD-005; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 1; CCR4-NOT transcription complex, subunit 1; Negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1H
Gene Aliases: 6030411K04Rik; AA815922; AD-005; CDC39; CNOT1; D830048B13; KIAA1007; NOT1; NOT1H; RGD1308009
UniProt ID: (Human) A5YKK6, (Mouse) Q6ZQ08
Entrez Gene ID: (Human) 23019, (Rat) 291841, (Mouse) 234594
negative regulation of transcription from RNA polymerase II promoter
trophectodermal cell differentiation
biological_process
positive regulation of cytoplasmic mRNA processing body assembly
negative regulation of translation
negative regulation of intracellular estrogen receptor signaling pathway
gene silencing by miRNA
negative regulation of retinoic acid receptor signaling pathway
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
regulation of stem cell population maintenance
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of translation
multicellular organism development
gene silencing by RNA
nuclear-transcribed mRNA poly(A) tail shortening
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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