Proteintech

CNOT1 Monoclonal Antibody (1A10A11)

CNOT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

CNOT1 Antibody (66507-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 66507-1-IG (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

CNOT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66507-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A10A11

Immunogen

CNOT1 Fusion Protein Ag5623 (1-354 aa encoded by BC040523)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against a region from the C-terminal CNOT1 of human origin. CNOT1 exists as many isoforms. Isoform one is about 267 kDa and isoform four is about 173 kDa.

Immunogen sequence: MNLDSLSLA LSQISYLVDN LTKKNYRASQ QEIQHIVNRH GPEADRHLLR CLFSHVDFSG DGKSSGKDFH QTQFLIQECA LLITKPNFIS TLSYAIDNPL HYQKSLKPAP HLFAQLSKVL KLSKVQEVIF GLALLNSSSS DLRGFAAQFI KQKLPDLLRS YIDADVSGNQ EGGFQDIAIE VLHLLLSHLL FGQKGAFGVG QEQIDAFLKT LRRDFPQERC PVVLAPLLYP EKRDILMDRI LPDSGGVAKT MMESSLADFM QEVGYGFCAS IEECRNIIVQ FGVREVTAAQ VARVLGMMAR THSGLTDGIP LQSISAPGSG IWSDGKDKSD GAQAHTWNVE VLIDVLKELN PSLNF (1-354 aa encoded by BC040523)

Target Information

Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Cell reports

Curdy N,Lanvin O,Cerapio JP,Pont F,Tosolini M,Sarot E,Valle C,Saint-Laurent N,Lhuillier E,Laurent C,Fournié JJ,Franchini DM

Tue Mar 28 00:00:00 UTC 2023

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Cell reports

Curdy N,Lanvin O,Cerapio JP,Pont F,Tosolini M,Sarot E,Valle C,Saint-Laurent N,Lhuillier E,Laurent C,Fournié JJ,Franchini DM

Tue Mar 28 00:00:00 UTC 2023

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Nature communications

Matkovic R,Morel M,Lanciano S,Larrous P,Martin B,Bejjani F,Vauthier V,Hansen MMK,Emiliani S,Cristofari G,Gallois-Montbrun S,Margottin-Goguet F

Mon Jan 10 00:00:00 UTC 2022

Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.

Molecular cell

Sarshad AA,Juan AH,Muler AIC,Anastasakis DG,Wang X,Genzor P,Feng X,Tsai PF,Sun HW,Haase AD,Sartorelli V,Hafner M

Thu Sep 20 00:00:00 UTC 2018

Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.

Molecular cell

Sarshad AA,Juan AH,Muler AIC,Anastasakis DG,Wang X,Genzor P,Feng X,Tsai PF,Sun HW,Haase AD,Sartorelli V,Hafner M

Thu Sep 20 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: adrenal gland protein AD-005; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 1; CCR4-NOT transcription complex, subunit 1; Negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1H

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Gene Aliases: 6030411K04Rik; AA815922; AD-005; CDC39; CNOT1; D830048B13; KIAA1007; NOT1; NOT1H; RGD1308009

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UniProt ID: (Human) A5YKK6, (Mouse) Q6ZQ08

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Entrez Gene ID: (Human) 23019, (Rat) 291841, (Mouse) 234594

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Function(s)

poly(A)-specific ribonuclease activity protein binding protein domain specific binding estrogen receptor binding protein complex scaffold retinoic acid receptor binding poly(A) RNA binding armadillo repeat domain binding molecular_function

Process(es)

negative regulation of transcription from RNA polymerase II promoter trophectodermal cell differentiation biological_process positive regulation of cytoplasmic mRNA processing body assembly negative regulation of translation negative regulation of intracellular estrogen receptor signaling pathway gene silencing by miRNA negative regulation of retinoic acid receptor signaling pathway positive regulation of nuclear-transcribed mRNA poly(A) tail shortening positive regulation of mRNA catabolic process RNA phosphodiester bond hydrolysis, exonucleolytic positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay regulation of stem cell population maintenance transcription, DNA-templated regulation of transcription, DNA-templated regulation of translation multicellular organism development gene silencing by RNA nuclear-transcribed mRNA poly(A) tail shortening DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CNOT1 Monoclonal Antibody (1A10A11)

CNOT1 Antibody (66507-1-IG) in ICC/IF

Product Details

66507-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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