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CNOT2 Polyclonal Antibody

CNOT2 Antibody in Immunocytochemistry (ICC/IF)

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CNOT2 Antibody (10313-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 10313-1-AP (CNOT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

10313-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CNOT2 Fusion Protein Ag0347 (336-540 aa encoded by BC002597)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QVLPDGRVT NIPQGMVTDQ FGMIGLLTFI RAAETDPGMV HLALGSDLTT LGLNLNSPEN LYPKFASPWA SSPCRPQDID FHVPSEYLTN IHIRDKLAAI KLGRYGEDLL FYLYYMNGGD VLQLLAAVEL FNRDWRYHKE ERVWITRAPG MEPTMKTNTY ERGTYYFFDC LNWRKVAKEF HLEYDKLEER PHLPSTFNYN PAQQAF (336-540 aa encoded by BC002597)

Target Information

CCR4-NOT Transcription Complex 2 (CNOT2) is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. CNOT2 is required for the CCR4-NOT complex structural integrity. It can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. CNOT2 is involved in the maintenance of embryonic stem (ES) cell identity. (UniProt)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.

RNA biology

Mostafa D,Takahashi A,Yanagiya A,Yamaguchi T,Abe T,Kureha T,Kuba K,Kanegae Y,Furuta Y,Yamamoto T,Suzuki T

Sun Mar 01 00:00:00 UTC 2020

CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.

Stem cell reports

Zheng X,Yang P,Lackford B,Bennett BD,Wang L,Li H,Wang Y,Miao Y,Foley JF,Fargo DC,Jin Y,Williams CJ,Jothi R,Hu G

Tue Nov 08 00:00:00 UTC 2016

CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.

Stem cell reports

Zheng X,Yang P,Lackford B,Bennett BD,Wang L,Li H,Wang Y,Miao Y,Foley JF,Fargo DC,Jin Y,Williams CJ,Jothi R,Hu G

Tue Nov 08 00:00:00 UTC 2016

Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.

Stem cells and development

Kamon M,Katano M,Hiraki-Kamon K,Hishida T,Nakachi Y,Mizuno Y,Okazaki Y,Suzuki A,Hirasaki M,Ueda A,Nishimoto M,Kato H,Okuda A

Mon Sep 15 00:00:00 UTC 2014

Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.

Stem cells and development

Kamon M,Katano M,Hiraki-Kamon K,Hishida T,Nakachi Y,Mizuno Y,Okazaki Y,Suzuki A,Hirasaki M,Ueda A,Nishimoto M,Kato H,Okuda A

Mon Sep 15 00:00:00 UTC 2014

Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.

Stem cells (Dayton, Ohio)

Zheng X,Dumitru R,Lackford BL,Freudenberg JM,Singh AP,Archer TK,Jothi R,Hu G

Tue May 01 00:00:00 UTC 2012

Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.

Stem cells (Dayton, Ohio)

Zheng X,Dumitru R,Lackford BL,Freudenberg JM,Singh AP,Archer TK,Jothi R,Hu G

Tue May 01 00:00:00 UTC 2012

Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.

Stem cells (Dayton, Ohio)

Zheng X,Dumitru R,Lackford BL,Freudenberg JM,Singh AP,Archer TK,Jothi R,Hu G

Tue May 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CCR4-associated factor 2; CCR4-NOT transcription complex subunit 2; CCR4-NOT transcription complex, subunit 2; negative regulator of transcription 2

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Gene Aliases: 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650; CDC36; CNOT2; HSPC131; MSTP046; NOT2; NOT2H

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UniProt ID: (Human) Q9NZN8, (Mouse) Q8C5L3

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Entrez Gene ID: (Human) 4848, (Rat) 299805, (Mouse) 72068

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Function(s)

RNA polymerase II transcription cofactor activity RNA polymerase II transcription corepressor binding poly(A)-specific ribonuclease activity protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter trophectodermal cell differentiation positive regulation of cytoplasmic mRNA processing body assembly negative regulation of intracellular estrogen receptor signaling pathway RNA phosphodiester bond hydrolysis, exonucleolytic regulation of stem cell population maintenance transcription, DNA-templated regulation of transcription, DNA-templated regulation of translation multicellular organism development gene silencing by RNA nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay nuclear-transcribed mRNA poly(A) tail shortening regulation of transcription from RNA polymerase II promoter DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest negative regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CNOT2 Polyclonal Antibody

CNOT2 Antibody (10313-1-AP) in ICC/IF

Product Details

10313-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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