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Invitrogen
CNOT7 Recombinant Rabbit Monoclonal Antibody (2H45L49)
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FIGURE: 1 / 3
CNOT7 Antibody (703786) in WB
Western blot was performed using Anti-CNOT7 Rabbit Recombinant Monoclonal Antibody (Product # 703786) and a 32 kDa band corresponding to CNOT7 was observed across the cell lines tested. Modified Whole cell extracts (1% SDS) (30 µg lysate) of Hep G2 (Lane 1), Jurkat (Lane 2), LNCaP (Lane 3), MOLT-4 (Lane 4) and THP-1 (Lane 5) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Pr... View More
Product Details
703786
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H45L49
Immunogen
Protein corresponding to human CNOT7 [aa1-aa285]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809250
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Bovine.
Target Information
CNOT7 (CCR4-NOT transcription complex subunit 7) has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. CNOT7 is a catalytic component of the CCR4-NOT complex, which is one of the major cellular mRNA deadenylases, and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. During miRNA-mediated repression, the complex seems to act as a transcriptional repressor during translational initiation, as well. Additional complex functions may be a consequence of its influence on mRNA expression. CNOT7 associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BTG1-binding factor 1; CAF-1; Caf1a; carbon catabolite repressor protein (CCR4)-associative factor 1; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 7
Gene Aliases: CAF-1; CAF1; Caf1a; CNOT7; hCAF-1
UniProt ID: (Human) Q9UIV1
Entrez Gene ID: (Human) 29883
nuclear-transcribed mRNA poly(A) tail shortening
deadenylation-dependent decapping of nuclear-transcribed mRNA
carbohydrate metabolic process
transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
signal transduction
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of gene expression
gene silencing by RNA
cytoplasmic mRNA processing body assembly
gene silencing by miRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
positive regulation of transcription from RNA polymerase II promoter
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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