Proteintech

CNOT9 Polyclonal Antibody

CNOT9 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

CNOT9 Antibody (22503-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 22503-1-AP (RQCD1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IGG (red).

CNOT9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CNOT9 Antibody in Immunoprecipitation (IP)

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Product Details

22503-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CNOT9 Fusion Protein Ag18197 (28-269 aa encoded by BC007102)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MHSLATAAP VPTTLAQVDR EKIYQWINEL SSPETRENAL LELSKKRESV PDLAPMLWHS FGTIAALLQE IVNIYPSINP PTLTAHQSNR VCNALALLQC VASHPETRSA FLAAHIPLFL YPFLHTVSKT RPFEYLRLTS LGVTGALVKT DEQEVINFLL TTEIIPLCLR IMESGSELSK TVATFILQKI LLDDTGLAYI CQTYERFSHV AMILGKMVLQ LSKEPSARLL KHVVRCYLRL SDN (28-269 aa encoded by BC007102)

Target Information

This gene encodes a member of the highly conserved RCD1 protein family. The encoded protein is a transcriptional cofactor and a core protein of the CCR4-NOT complex. It may be involved in signal transduction as well as retinoic acid-regulated cell differentiation and development. Alternatively spliced transcript variants have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Post-translational control of beige fat biogenesis by PRDM16 stabilization.

Nature

Wang Q,Li H,Tajima K,Verkerke ARP,Taxin ZH,Hou Z,Cole JB,Li F,Wong J,Abe I,Pradhan RN,Yamamuro T,Yoneshiro T,Hirschhorn JN,Kajimura S

Thu Sep 01 00:00:00 UTC 2022

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Nature communications

Matkovic R,Morel M,Lanciano S,Larrous P,Martin B,Bejjani F,Vauthier V,Hansen MMK,Emiliani S,Cristofari G,Gallois-Montbrun S,Margottin-Goguet F

Mon Jan 10 00:00:00 UTC 2022

The Conserved CNOT1 Interaction Motif of Tristetraprolin Regulates ARE-mRNA Decay Independently of the p38 MAPK-MK2 Kinase Pathway.

Molecular and cellular biology

Carreño A,Lykke-Andersen J

Thu Sep 15 00:00:00 UTC 2022

The Conserved CNOT1 Interaction Motif of Tristetraprolin Regulates ARE-mRNA Decay Independently of the p38 MAPK-MK2 Kinase Pathway.

Molecular and cellular biology

Carreño A,Lykke-Andersen J

Thu Sep 15 00:00:00 UTC 2022

The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.

iScience

Zou L,Moch C,Graille M,Chapat C

Fri Jul 15 00:00:00 UTC 2022

Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex.

Genes

Munakata F,Suzawa M,Ui-Tei K

Sat Feb 13 00:00:00 UTC 2021

RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.

Nature communications

Poetz F,Corbo J,Levdansky Y,Spiegelhalter A,Lindner D,Magg V,Lebedeva S,Schweiggert J,Schott J,Valkov E,Stoecklin G

Thu Dec 09 00:00:00 UTC 2021

RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.

Nature communications

Poetz F,Corbo J,Levdansky Y,Spiegelhalter A,Lindner D,Magg V,Lebedeva S,Schweiggert J,Schott J,Valkov E,Stoecklin G

Thu Dec 09 00:00:00 UTC 2021

Phase Transitions in the Assembly and Function of Human miRISC.

Cell

Sheu-Gruttadauria J,MacRae IJ

Thu May 03 00:00:00 UTC 2018

Phase Transitions in the Assembly and Function of Human miRISC.

Cell

Sheu-Gruttadauria J,MacRae IJ

Thu May 03 00:00:00 UTC 2018

Translational control of ERK signaling through miRNA/4EHP-directed silencing.

eLife

Jafarnejad SM,Chapat C,Matta-Camacho E,Gelbart IA,Hesketh GG,Arguello M,Garzia A,Kim SH,Attig J,Shapiro M,Morita M,Khoutorsky A,Alain T,Gkogkas CG,Stern-Ginossar N,Tuschl T,Gingras AC,Duchaine TF,Sonenberg N

Wed Feb 07 00:00:00 UTC 2018

Translational control of ERK signaling through miRNA/4EHP-directed silencing.

eLife

Wed Feb 07 00:00:00 UTC 2018

Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.

Nucleic acids research

Mauri M,Kirchner M,Aharoni R,Ciolli Mattioli C,van den Bruck D,Gutkovitch N,Modepalli V,Selbach M,Moran Y,Chekulaeva M

Wed Jan 25 00:00:00 UTC 2017

Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.

Nucleic acids research

Mauri M,Kirchner M,Aharoni R,Ciolli Mattioli C,van den Bruck D,Gutkovitch N,Modepalli V,Selbach M,Moran Y,Chekulaeva M

Wed Jan 25 00:00:00 UTC 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 UTC 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 UTC 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 UTC 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 UTC 2017

Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.

Molecular cell

Mathys H,Basquin J,Ozgur S,Czarnocki-Cieciura M,Bonneau F,Aartse A,Dziembowski A,Nowotny M,Conti E,Filipowicz W

Thu Jun 05 00:00:00 UTC 2014

Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.

Molecular cell

Mathys H,Basquin J,Ozgur S,Czarnocki-Cieciura M,Bonneau F,Aartse A,Dziembowski A,Nowotny M,Conti E,Filipowicz W

Thu Jun 05 00:00:00 UTC 2014

A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.

Molecular cell

Chen Y,Boland A,Kuzuoğlu-Öztürk D,Bawankar P,Loh B,Chang CT,Weichenrieder O,Izaurralde E

Thu Jun 05 00:00:00 UTC 2014

A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.

Molecular cell

Chen Y,Boland A,Kuzuoğlu-Öztürk D,Bawankar P,Loh B,Chang CT,Weichenrieder O,Izaurralde E

Thu Jun 05 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: cancer/testis antigen 129; CCR4-NOT transcription complex subunit 9; cell differentiation protein RCD1 homolog; Cell differentiation protein RQCD1 homolog; EPO-induced protein FL10; protein involved in sexual development; Rcd-1; rcd1 (required for cell differentiation) homolog 1; RCD1 required for cell differentiation1 homolog

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Gene Aliases: 2610007F23Rik; AI593551; CAF40; CNOT9; CT129; Fl10; RCD-1; RCD1; RQCD1

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UniProt ID: (Human) Q92600, (Mouse) Q9JKY0, (Rat) Q5PQL2

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Entrez Gene ID: (Human) 9125, (Mouse) 58184, (Rat) 301513

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Function(s)

protein binding protein domain specific binding protein homodimerization activity

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated regulation of translation cytokine-mediated signaling pathway gene silencing by RNA negative regulation of intracellular estrogen receptor signaling pathway positive regulation of ligand-dependent nuclear receptor transcription coactivator activity nuclear-transcribed mRNA poly(A) tail shortening DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest sex differentiation negative regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CNOT9 Polyclonal Antibody

CNOT9 Antibody (22503-1-AP) in ICC/IF

Product Details

22503-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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