Invitrogen

CNR2 Polyclonal Antibody

7 Published Figures

11 References

View all (14) CNR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (7)

CNR2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

CNR2 Antibody (PA1-746A) in ICC/IF

Immunolocalization of CB2 in AtT20 cells transfected with the rat CB2 gene using Product # PA1-746A.

CNR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CNR2 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-746A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GST fusion protein containing the first 32 amino acid residues from Rat CB2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2082908

Product Specific Information

PA1-746A detects cannabinoid receptor 2 (CB2) from human and rat tissues as well as transfected rat CB2.

PA1-746A has been successfully used in WB, immunocytochemistry, immunohistochemistry (paraffin) and FACS procedures. Immunocytochemical staining of CB2 into AtT20 cells transfected with the rat CB2 gene with PA1-746 yields a pattern consistent with plasma membrane staining.

Target Information

Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spinal cannabinoid receptor 2 activation alleviates neuropathic pain by regulating microglia and suppressing P2X7 receptor.

Frontiers in molecular neuroscience

Zhou Y,Xu Y,Yang J,Yu Z,Wang W,Yuan M,Wang Y,Bai Q,Li Z

PA1-746A was used in Western Blotting to provide new insights into the neuroprotective mechanism of CB2R activation.

Tue Mar 28 00:00:00 UTC 2023

Synthetic Cannabinoid Agonist WIN 55212-2 Targets Proliferation, Angiogenesis, and Apoptosis via MAPK/AKT Signaling in Human Endometriotic Cell Lines and a Murine Model of Endometriosis.

Frontiers in reproductive health

Lingegowda H,Miller JE,Marks RM,Symons LK,Alward T,Lomax AE,Koti M,Tayade C

Published figure using CNR2 polyclonal antibody (Product # PA1-746A) in Immunohistochemistry

Sat Oct 29 00:00:00 UTC 2022

Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance.

Brain structure & function

Wagner F,Bernard R,Derst C,French L,Veh RW

PA1746A was used in immunohistochemistry to determine if medial or lateral habenula or their subnuclei display field-characteristic gene products

Thu Dec 01 00:00:00 UTC 2016

Neuronal expression of CB2 cannabinoid receptor mRNAs in the mouse hippocampus.

Neuroscience

Li Y,Kim J

PA1-746A was used in Western Blotting to study the mechanisms of CB2 cannabinoid receptor functions in the brain.

Thu Dec 17 00:00:00 UTC 2015

Islet cannabinoid receptors: cellular distribution and biological function.

Pancreas

Flores LE,Alzugaray ME,Cubilla MA,Raschia MA,Del Zotto HH,Román CL,Suburo AM,Gagliardino JJ

PA1-746A was used in immunohistochemistry to study the expression of cannabinoid receptors in rat islets and their physiological significance

Tue Oct 01 00:00:00 UTC 2013

Prevention of paclitaxel-induced neuropathy through activation of the central cannabinoid type 2 receptor system.

Anesthesia and analgesia

Naguib M,Xu JJ,Diaz P,Brown DL,Cogdell D,Bie B,Hu J,Craig S,Hittelman WN

PA1-746A was used in Immunohistochemistry on frozen tissues to examine the potential of cannabinoid type 2 agonists in preventing paclitaxel-induced neuropathy.

Tue May 01 00:00:00 UTC 2012

Overexpression of cannabinoid CB2 receptor in the brain induces hyperglycaemia and a lean phenotype in adult mice.

Journal of neuroendocrinology

Romero-Zerbo SY,Garcia-Gutierrez MS,Suárez J,Rivera P,Ruz-Maldonado I,Vida M,Rodriguez de Fonseca F,Manzanares J,Bermúdez-Silva FJ

PA1-746A was used in immunohistochemistry to study the effects of CB2 receptor overexpression in murine brain on body mass and glucose metabolism

Wed Aug 01 00:00:00 UTC 2012

Overexpression of cannabinoid CB2 receptor in the brain induces hyperglycaemia and a lean phenotype in adult mice.

Journal of neuroendocrinology

Romero-Zerbo SY,Garcia-Gutierrez MS,Suárez J,Rivera P,Ruz-Maldonado I,Vida M,Rodriguez de Fonseca F,Manzanares J,Bermúdez-Silva FJ

PA1-746A was used in immunohistochemistry to study the effects of CB2 receptor overexpression in murine brain on body mass and glucose metabolism

Wed Aug 01 00:00:00 UTC 2012

Involvement of the endocannabinoid system in periodontal healing.

Biochemical and biophysical research communications

Kozono S,Matsuyama T,Biwasa KK,Kawahara K,Nakajima Y,Yoshimoto T,Yonamine Y,Kadomatsu H,Tancharoen S,Hashiguchi T,Noguchi K,Maruyama I

PA1-746A was used in immunohistochemistry to study the role of endocannabinoid receptors in periodontal healing

Fri Apr 16 00:00:00 UTC 2010

Cannabinoid receptors in conjunctival epithelium: identification and functional properties.

Investigative ophthalmology & visual science

Iribarne M,Torbidoni V,Julián K,Prestifilippo JP,Sinha D,Rettori V,Berra A,Suburo AM

PA1-746A was used in Immunohistochemistry to explore the presence and properties of cannabinoid receptors in conjunctival epithelial cells.

Wed Oct 01 00:00:00 UTC 2008

Dual effect of anandamide on rat placenta nitric oxide synthesis.

Placenta

Cella M,Leguizamón GF,Sordelli MS,Cervini M,Guadagnoli T,Ribeiro ML,Franchi AM,Farina MG

PA1-746A was used in western blot to study the effect of anandamide on nitric oxide synthesis in rat placenta

Fri Aug 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: Cannabinoid Receptor 2; cannabinoid receptor 2 (macrophage); CB-2; CB2 receptor; CB2R; CNR3; CX5; HCB2; HGNC:2160; OTTHUMP00000044841; peripheral cannabinoid receptor; rCB2; RP11-4M23.1; testis-dominant CNR2 isoform CB2

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Gene Aliases: CB-2; CB2; CB2A; CB2B; CB2C; CNR2; CNR2C; CX5

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UniProt ID: (Human) P34972, (Rat) Q9QZN9

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Entrez Gene ID: (Human) 1269, (Rat) 57302

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Function(s)

cannabinoid receptor activity G-protein coupled receptor activity

Process(es)

response to amphetamine inflammatory response G-protein coupled receptor signaling pathway sensory perception of pain negative regulation of synaptic transmission, GABAergic response to lipopolysaccharide negative regulation of mast cell activation cannabinoid signaling pathway negative regulation of action potential negative regulation of inflammatory response negative regulation of nitric-oxide synthase activity immune response G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger

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CNR2 Polyclonal Antibody

CNR2 Antibody (PA1-746A) in ICC/IF

Product Details

PA1-746A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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