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CNTF Antibody (PA5-95172) in IHC (P)
Immunohistochemical analysis of CNTF in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CNTF antibody (Product # PA5-95172) overnight at 4°C. Biotinylated goat anti-rabbit... View More
Product Details
PA5-95172
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CNTF recombinant protein (Position: A2-M200).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806977
Product Specific Information
Human CNTF shares 83% and 84% amino acid (aa) sequence identity with mouse and rat CNTF, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - IHC: human intestinal cancer tissue, human lung cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ciliary neurotrophic factor; CNTF; H-CNTF
Gene Aliases: CNTF; HCNTF
UniProt ID: (Human) P26441
Entrez Gene ID: (Human) 1270
signal transduction
positive regulation of cell proliferation
positive regulation of gene expression
growth
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of neuron apoptotic process
negative regulation of photoreceptor cell differentiation
regulation of retinal cell programmed cell death
astrocyte activation
muscle organ morphogenesis
neuron development
positive regulation of axon regeneration
ciliary neurotrophic factor-mediated signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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