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COBRA1 Antibody (PA5-79728) in WB
Western blot analysis of COBRA1 in, Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane ... View More
Product Details
PA5-79728
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human COBRA1 recombinant protein (Position: M1-E252).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746843
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, mouse kidney tissue, human MDA-MB-231 whole cell.
Target Information
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2), either NELFC or NELFD (TH1L), and NELFE (RDBP), a heterodimer of SPT4 (SUPT4H1), to cause transcriptional pausing of RNA polymerase II (Narita et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cofactor of BRCA1; DKFZp586B0519; Negative elongation factor B; negative elongation factor complex member B, Cobra1; negative elongation factor protein B; NELF-B; ortholog of human cofactor of BRCA1 (COBRA1); RP13-122B23.3
Gene Aliases: A730008L03Rik; AB041607; AI663983; COBRA1; KIAA1182; MNCb-5210; NELF-B; NELFB; RGD1307832
UniProt ID: (Human) Q8WX92, (Mouse) Q8C4Y3
Entrez Gene ID: (Human) 25920, (Mouse) 58202, (Rat) 311796
transcription, DNA-templated
regulation of transcription, DNA-templated
negative regulation of transcription, DNA-templated
translational elongation
biological_process
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
negative regulation of transcription elongation from RNA polymerase II promoter
positive regulation of viral transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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