Proteintech

COL1A2 Monoclonal Antibody (3D5E8)

COL1A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

COL1A2 Antibody (66761-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66761-1-IG (COL1A2 antibody) at dilution of 1:4800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

66761-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 42 publications 42 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2400-1:9600

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D5E8

Immunogen

COL1A2 Fusion Protein Ag6281 (1017-1366 aa encoded by BC054498)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RGLPGLKGH NGLQGLPGIA GHHGDQGAPG SVGPAGPRGP AGPSGPAGKD GRTGHPGTVG PAGIRGPQGH QGPAGPPGPP GPPGPPGVSG GGYDFGYDGD FYRADQPRSA PSLRPKDYEV DATLKSLNNQ IETLLTPEGS RKNPARTCRD LRLSHPEWSS GYYWIDPNQG CTMDAIKVYC DFSTGETCIR AQPENIPAKN WYRSSKDKKH VWLGETINAG SQFEYNVEGV TSKEMATQLA FMRLLANYAS QNITYHCKNS IAYMDEETGN LKKAVILQGS NDVELVAEGN SRFTYTVLVD GCSKKTNEWG KTIIEYKTNK PSRLPFLDIA PLDIGGADQE FFVDIGPVCF K (1017-1366 aa encoded by BC054498)

Target Information

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy.

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Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-2 type I collagen; COL1; Collagen alpha-2(I) chain; collagen COL1A2; Collagen I; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 2; osteogenesis imperfecta; procollagen, type I, alpha 2; type I procollagen

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Gene Aliases: AA960264; AI325291; Col1a-2; COL1A2; Cola-2; Cola2; OI4; oim

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UniProt ID: (Human) P08123, (Mouse) Q01149, (Rat) P02466

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Entrez Gene ID: (Human) 1278, (Pig) 100626716, (Mouse) 12843, (Rat) 84352

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Function(s)

extracellular matrix structural constituent protein binding protein binding, bridging identical protein binding SMAD binding metal ion binding platelet-derived growth factor binding molecular_function

Process(es)

skeletal system development blood vessel development transforming growth factor beta receptor signaling pathway Rho protein signal transduction blood coagulation regulation of blood pressure platelet activation extracellular matrix organization collagen fibril organization collagen catabolic process odontogenesis skin morphogenesis regulation of immune response leukocyte migration protein heterotrimerization cellular response to amino acid stimulus cellular response to retinoic acid cellular response to organic substance response to norepinephrine cellular response to thyroid hormone stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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COL1A2 Monoclonal Antibody (3D5E8)

COL1A2 Antibody (66761-1-IG) in IHC (P)

Product Details

66761-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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