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Invitrogen
COL6A1 Polyclonal Antibody
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FIGURE: 1 / 1
COL6A1 Antibody (PA5-121228) in WB
Western blot analysis of COL6A1 in extracts of various cell lines. Samples were incubated with COL6A1 Polyclonal antibody (Product # PA5-121228) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-121228
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-250 of human Collagen VI/Collagen VI/COL6A1 (NP_0018392)
Conjugate
Form
Liquid
Concentration
1.37 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914800
Product Specific Information
Positive test controls include: SKOV3, HepG2, HT-1080, Mouse heart, Mouse lung, Mouse pancreas. The target is usually found in the following locations: Secreted, extracellular matrix, extracellular space.
Immunogen sequence: AQDEPETPRA VAFQDCPVDL FFVLDTSESV ALRLKPYGAL VDKVKSFTKR FIDNLRDRYY RCDRNLVWNA GALHYSDEVE IIQGLTRMPG GRDALKSSVD AVKYFGKGTY TDCAIKKGLE QLLVGGSHLK ENKYLIVVTD GHPLEGYKEP CGGLEDAVNE AKHLGVKVFS VAITPDHLEP RLSIIATDHT YRRNFTAADW GQSRDAEEAI SQTIDTIVDM IKNNVEQVCC SF
Target Information
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (typesI, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 1 (VI) chain (61 AA); Collagen alpha-1(VI) chain; collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1; procollagen, type VI, alpha 1
Gene Aliases: AI747156; BTHLM1; Col6a-1; COL6A1; OPLL; UCHMD1
UniProt ID: (Human) P12109, (Mouse) Q04857
Entrez Gene ID: (Human) 1291, (Mouse) 12833
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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