Proteintech

COMMD1 Polyclonal Antibody

COMMD1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

COMMD1 Antibody (11938-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 11938-1-AP (COMMD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

COMMD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COMMD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11938-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COMMD1 Fusion Protein Ag2535 (1-190 aa encoded by BC022046)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAAGELEGG KPLSGLLNAL AQDTFHGYPG ITEELLRSQL YPEVPPEEFR PFLAKMRGIL KSIASADMDF NQLEAFLTAQ TKKQGGITSD QAAVISKFWK SHKTKIRESL MNQSRWNSGL RGLSWRVDGK SQSRHSAQIH TPVAIIELEL GKYGQESEFL CLEFDEVKVN QILKTLSEVE ESISTLISQP N (1-190 aa encoded by BC022046)

Target Information

Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activiy and dissocoiates CAND1 from CUL1 and CUL2. Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Involved in the regulation of membrane expression and ubiquitination of SLC12A2. Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes. Promotes CFTR cell surface expression through regulation of its ubiquitination. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans- Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recruitment of the SNX17-Retriever recycling pathway regulates synaptic function and plasticity.

The Journal of cell biology

Rivero-Ríos P,Tsukahara T,Uygun T,Chen A,Chavis GD,Giridharan SSP,Iwase S,Sutton MA,Weisman LS

Mon Jul 03 00:00:00 UTC 2023

Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.

Cell

Healy MD,McNally KE,Butkovič R,Chilton M,Kato K,Sacharz J,McConville C,Moody ERR,Shaw S,Planelles-Herrero VJ,Yadav SKN,Ross J,Borucu U,Palmer CS,Chen KE,Croll TI,Hall RJ,Caruana NJ,Ghai R,Nguyen THD,Heesom KJ,Saitoh S,Berger I,Schaffitzel C,Williams TA,Stroud DA,Derivery E,Collins BM,Cullen PJ

Thu May 11 00:00:00 UTC 2023

Chrysin protects against cerebral ischemia-reperfusion injury in hippocampus via restraining oxidative stress and transition elements.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Shang J,Jiao J,Yan M,Wang J,Li Q,Shabuerjiang L,Lu Y,Song Q,Bi L,Huang G,Zhang X,Wen Y,Cui Y,Wu K,Li G,Wang P,Liu X

Mon May 01 00:00:00 UTC 2023

Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex.

Science immunology

Shirai T,Nakai A,Ando E,Fujimoto J,Leach S,Arimori T,Higo D,van Eerden FJ,Tulyeu J,Liu YC,Okuzaki D,Murayama MA,Miyata H,Nunomura K,Lin B,Tani A,Kumanogoh A,Ikawa M,Wing JB,Standley DM,Takagi J,Suzuki K

Fri Mar 31 00:00:00 UTC 2023

Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.

Molecular metabolism

Yang L,Fye MA,Yang B,Tang Z,Zhang Y,Haigh S,Covington BA,Bracey K,Taraska JW,Kaverina I,Qu S,Chen W

Thu Sep 01 00:00:00 UTC 2022

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

EMBO reports

Chen Y,Wu X,Zhang J,Pan G,Wang X,Guo X,Wang J,Cui X,Gao H,Cheng M,Yang J,Zhang C,Jiang F

Thu Feb 03 00:00:00 UTC 2022

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

eLife

Giridharan SSP,Luo G,Rivero-Rios P,Steinfeld N,Tronchere H,Singla A,Burstein E,Billadeau DD,Sutton MA,Weisman LS

Tue Jan 18 00:00:00 UTC 2022

The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.

Neurobiology of disease

Bakkar N,Starr A,Rabichow BE,Lorenzini I,McEachin ZT,Kraft R,Chaung M,Macklin-Isquierdo S,Wingfield T,Carhart B,Zahler N,Chang WH,Bassell GJ,Betourne A,Boulis N,Alworth SV,Ichida JK,August PR,Zarnescu DC,Sattler R,Bowser R

Mon Feb 01 00:00:00 UTC 2021

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Theranostics

Lin L,Chen S,Wang H,Gao B,Kallakury B,Bhuvaneshwar K,Cahn K,Gusev Y,Wang X,Wu Y,Marshall JL,Zhi X,He AR

Thu Jul 29 00:00:00 UTC 2021

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Nature communications

Zhu Y,Feng F,Hu G,Wang Y,Yu Y,Zhu Y,Xu W,Cai X,Sun Z,Han W,Ye R,Qu D,Ding Q,Huang X,Chen H,Xu W,Xie Y,Cai Q,Yuan Z,Zhang R

Thu Feb 11 00:00:00 UTC 2021

The COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.

Circulation research

Fedoseienko A,Wijers M,Wolters JC,Dekker D,Smit M,Huijkman N,Kloosterhuis N,Klug H,Schepers A,Willems van Dijk K,Levels JHM,Billadeau DD,Hofker MH,van Deursen J,Westerterp M,Burstein E,Kuivenhoven JA,van de Sluis B

Fri Jun 08 00:00:00 UTC 2018

The COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.

Circulation research

Fri Jun 08 00:00:00 UTC 2018

The COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.

Circulation research

Fri Jun 08 00:00:00 UTC 2018

The COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.

Circulation research

Fri Jun 08 00:00:00 UTC 2018

HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.

The Journal of cell biology

MacDonald E,Brown L,Selvais A,Liu H,Waring T,Newman D,Bithell J,Grimes D,Urbé S,Clague MJ,Zech T

Mon Jul 02 00:00:00 UTC 2018

HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.

The Journal of cell biology

MacDonald E,Brown L,Selvais A,Liu H,Waring T,Newman D,Bithell J,Grimes D,Urbé S,Clague MJ,Zech T

Mon Jul 02 00:00:00 UTC 2018

COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.

Cell reports