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COMT Polyclonal Antibody

COMT Antibody in Immunocytochemistry (ICC/IF)

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COMT Antibody (14754-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using COMT antibody 14754-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

COMT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COMT Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14754-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COMT Fusion Protein Ag6502 (90-271 aa encoded by BC000419)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MNVGDKKGK IVDAVIQEHQ PSVLLELGAY CGYSAVRMAR LLSPGARLIT IEINPDCAAI TQRMVDFAGM KDKVTLVVGA SQDIIPQLKK KYDVDTLDMV FLDHWKDRYL PDTLLLEECG LLRKGTVLLA DNVICPGAPD FLAHVRGSSC FECTHYQSFL EYREVVDGLE KAIYKGPGSE AGP (90-271 aa encoded by BC000419)

Target Information

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic Modulation of BET1L Confers Colorectal Cancer Susceptibility by Reducing miRNA Binding and m6A Modification.

Cancer research

Li S,Du M,Xu K,Ben S,Zhu T,Guo M,Xin J,Zhu L,Gu D,Zhang Z,Wang M

Wed Jul 05 00:00:00 UTC 2023

Mechanistic analysis of erectile dysfunction in a depression rat model.

The Journal of international medical research

Hong ZM,Chen ZL,Feng JL,Wang SJ,Qiu JF,Zeng YL,Wang Q,Wang JS

Sun May 01 00:00:00 UTC 2022

Vitamin D attenuated 6-OHDA-induced behavioural deficits, dopamine dysmetabolism, oxidative stress, and neuro-inflammation in mice.

Nutritional neuroscience

Bayo-Olugbami A,Nafiu AB,Amin A,Ogundele OM,Lee CC,Owoyele BV

Fri Apr 01 00:00:00 UTC 2022

Entacapone alleviates acute kidney injury by inhibiting ferroptosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yang J,Sun X,Huang N,Li P,He J,Jiang L,Zhang X,Han S,Xin H

Fri Jul 01 00:00:00 UTC 2022

Mechanism of Catechol-O-methyltransferase Regulating Orofacial Pain Induced by Tooth Movement.

BioMed research international

Zhou Y,Song Z,Chen S,Yao F,Liu J,Ouyang Z,Liao Z

Fri Jan 21 00:00:00 UTC 2022

Mechanism of Catechol-O-methyltransferase Regulating Orofacial Pain Induced by Tooth Movement.

BioMed research international

Zhou Y,Song Z,Chen S,Yao F,Liu J,Ouyang Z,Liao Z

Fri Jan 21 00:00:00 UTC 2022

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Aging

Wu X,Sheng H,Wang L,Xia P,Wang Y,Yu L,Lv W,Hu J

Fri Mar 26 00:00:00 UTC 2021

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Translational psychiatry

Guo Y,Zhu X,Zeng M,Qi L,Tang X,Wang D,Zhang M,Xie Y,Li H,Yang X,Chen D

Thu May 27 00:00:00 UTC 2021

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Aging

Wang D,Zhang H,Zeng M,Tang X,Zhu X,Guo Y,Qi L,Xie Y,Zhang M,Chen D

Fri Mar 26 00:00:00 UTC 2021

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.

Journal of proteomics

Prunotto M,Farina A,Lane L,Pernin A,Schifferli J,Hochstrasser DF,Lescuyer P,Moll S

Fri Apr 26 00:00:00 UTC 2013

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.

Journal of proteomics

Prunotto M,Farina A,Lane L,Pernin A,Schifferli J,Hochstrasser DF,Lescuyer P,Moll S

Fri Apr 26 00:00:00 UTC 2013

Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene.

Acta biochimica et biophysica Sinica

Liu Z,Luo X,Liu L,Zhao W,Guo S,Guo Y,Wang N,He H,Liao X,Ma W,Zhou H,Zhang T

Sun Dec 01 00:00:00 UTC 2013

Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene.

Acta biochimica et biophysica Sinica

Liu Z,Luo X,Liu L,Zhao W,Guo S,Guo Y,Wang N,He H,Liao X,Ma W,Zhou H,Zhang T

Sun Dec 01 00:00:00 UTC 2013

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Endocrinology

Saller S,Merz-Lange J,Raffael S,Hecht S,Pavlik R,Thaler C,Berg D,Berg U,Kunz L,Mayerhofer A

Thu Mar 01 00:00:00 UTC 2012

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Endocrinology

Saller S,Merz-Lange J,Raffael S,Hecht S,Pavlik R,Thaler C,Berg D,Berg U,Kunz L,Mayerhofer A

Thu Mar 01 00:00:00 UTC 2012

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Endocrinology

Saller S,Merz-Lange J,Raffael S,Hecht S,Pavlik R,Thaler C,Berg D,Berg U,Kunz L,Mayerhofer A

Thu Mar 01 00:00:00 UTC 2012

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Endocrinology

Saller S,Merz-Lange J,Raffael S,Hecht S,Pavlik R,Thaler C,Berg D,Berg U,Kunz L,Mayerhofer A

Thu Mar 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Catechol O-methyltransferase; catechol-O-methyltransferase 1; catechol-O-methyltransferase isoform; catecholamine-O-methyltransferase; epididymis secretory sperm binding protein Li 98n; OTTHUMP00000197749; OTTHUMP00000197750; testicular tissue protein Li 42

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Gene Aliases: COMT; Comt1; D16Wsu103e; D330014B15Rik; HEL-S-98n

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UniProt ID: (Human) P21964, (Mouse) O88587, (Rat) P22734

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Entrez Gene ID: (Human) 1312, (Mouse) 12846, (Rat) 24267

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Function(s)

magnesium ion binding protein binding methyltransferase activity O-methyltransferase activity catechol O-methyltransferase activity transferase activity metal ion binding

Process(es)

catecholamine metabolic process female pregnancy learning short-term memory estrogen metabolic process catechol-containing compound metabolic process cellular response to phosphate starvation methylation negative regulation of renal sodium excretion neurotransmitter catabolic process dopamine metabolic process dopamine catabolic process negative regulation of dopamine metabolic process response to pain multicellular organismal reproductive process negative regulation of smooth muscle cell proliferation positive regulation of homocysteine metabolic process regulation of sensory perception of pain response to organic cyclic compound response to lipopolysaccharide developmental process response to drug S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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COMT Polyclonal Antibody

COMT Antibody (14754-1-AP) in ICC/IF

Product Details

14754-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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