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Bethyl Laboratories
COP1/RFWD2 Polyclonal Antibody
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FIGURE: 1 / 1
COP1/RFWD2 Antibody (A300-895A) in IP
Detection of human COP1/RFWD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COP1/RFWD2 antibody (Product # A300-895A) (lot 2) used for IP at 6 µg per reaction. COP1/RFWD2 was also immunoprecipitated by rabbit anti-COP1/RFWD2 antibody A300-894A. For blotting immunoprecipitated COP1/RFWD2, A300-894A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes... View More
Product Details
A300-895A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of human Constitutive Photomorphogenic Protein-1/Ring Finger and WD Repeat Domain 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Constitutive photomorphogenesis protein 1 homolog; constitutive photomorphogenic protein 1; COP1D; E3 ubiquitin-protein ligase COP1; hCOP1; putative ubiquitin ligase COP1; ring finger and WD repeat domain 2, E3 ubiquitin protein ligase; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-type E3 ubiquitin transferase RFWD2
Gene Aliases: COP1; RFWD2; RNF200
UniProt ID: (Human) Q8NHY2
Entrez Gene ID: (Human) 64326
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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