Invitrogen

COX1 Monoclonal Antibody (COX 111)

11 Published Figures

20 References

View all (19) COX1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (11)

COX1 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 14

COX1 Antibody (35-8100) in IHC

Immunohistochemical staining of thyroid carcinoma tissue using COX-1 Monoclonal Antibody, Mouse (clone COX 111) (Product # 35-8100).

COX1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

COX1 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

35-8100

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 11 publications 11 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

COX 111

Immunogen

Ram testes.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533223

Target Information

PTGS1 (COX1, Cyclooxygenase 1) is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelium-specific deletion of amyloid-β precursor protein exacerbates endothelial dysfunction induced by aging.

Aging

d'Uscio LV,Katusic ZS

35-8100 was used in Western Blotting to support the concept that aging-induced upregulation of APP in vascular endothelium is an adaptive response designed to protect and preserve expression and function of eNOS.

Thu Aug 12 00:00:00 UTC 2021

Endothelium-specific deletion of amyloid-β precursor protein exacerbates endothelial dysfunction induced by aging.

Aging

d'Uscio LV,Katusic ZS

Thu Aug 12 00:00:00 UTC 2021

Mitochondrial AIF loss causes metabolic reprogramming, caspase-independent cell death blockade, embryonic lethality, and perinatal hydrocephalus.

Molecular metabolism

Delavallée L,Mathiah N,Cabon L,Mazeraud A,Brunelle-Navas MN,Lerner LK,Tannoury M,Prola A,Moreno-Loshuertos R,Baritaud M,Vela L,Garbin K,Garnier D,Lemaire C,Langa-Vives F,Cohen-Salmon M,Fernández-Silva P,Chrétien F,Migeotte I,Susin SA

35-8100 was used in Western Blotting to analyze, in a single paradigm, the cellular and developmental metabolic consequences of AIF loss and the subsequent oxidative phosphorylation (OXPHOS) dysfunction.

Thu Oct 01 00:00:00 UTC 2020

Mitochondrial AIF loss causes metabolic reprogramming, caspase-independent cell death blockade, embryonic lethality, and perinatal hydrocephalus.

Molecular metabolism

Thu Oct 01 00:00:00 UTC 2020

Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Q_pool.

Science advances

Calvo E,Cogliati S,Hernansanz-Agustín P,Loureiro-López M,Guarás A,Casuso RA,García-Marqués F,Acín-Pérez R,Martí-Mateos Y,Silla-Castro JC,Carro-Alvarellos M,Huertas JR,Vázquez J,Enríquez JA

35-8100 was used in Western Blot to study C57BL/6J mice harboring nonfunctional SCAF1, the full knockout for SCAF1, or the wild-type version of the protein and found that exercise performance is SCAF1 dependent.

Mon Jun 01 00:00:00 UTC 2020

Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Q_pool.

Science advances

Calvo E,Cogliati S,Hernansanz-Agustín P,Loureiro-López M,Guarás A,Casuso RA,García-Marqués F,Acín-Pérez R,Martí-Mateos Y,Silla-Castro JC,Carro-Alvarellos M,Huertas JR,Vázquez J,Enríquez JA

Mon Jun 01 00:00:00 UTC 2020

Vascular phenotype of amyloid precursor protein-deficient mice.

American journal of physiology. Heart and circulatory physiology

d'Uscio LV,Katusic ZS

35-8100 was used in Western Blotting to characterize the vascular phenotype of amyloid precursor protein (APP)-knockout (APP-/-) mice.

Sat Jun 01 00:00:00 UTC 2019

Mortaparib, a novel dual inhibitor of mortalin and PARP1, is a potential drug candidate for ovarian and cervical cancers.

Journal of experimental & clinical cancer research : CR

Putri JF,Bhargava P,Dhanjal JK,Yaguchi T,Sundar D,Kaul SC,Wadhwa R

35-8100 was used in Immunohistochemistry to report the discovery of Mortaparib as a dual inhibitor of mortalin and PARP1 and identify as a potential anticancer drug due to its tumor suppressor activity as a.

Thu Dec 19 00:00:00 UTC 2019

Mortaparib, a novel dual inhibitor of mortalin and PARP1, is a potential drug candidate for ovarian and cervical cancers.

Journal of experimental & clinical cancer research : CR

Putri JF,Bhargava P,Dhanjal JK,Yaguchi T,Sundar D,Kaul SC,Wadhwa R

Thu Dec 19 00:00:00 UTC 2019

Mortaparib, a novel dual inhibitor of mortalin and PARP1, is a potential drug candidate for ovarian and cervical cancers.

Journal of experimental & clinical cancer research : CR

Putri JF,Bhargava P,Dhanjal JK,Yaguchi T,Sundar D,Kaul SC,Wadhwa R

Thu Dec 19 00:00:00 UTC 2019

Resveratrol Promotes Mitochondrial Biogenesis and Protects against Seizure-Induced Neuronal Cell Damage in the Hippocampus Following Status Epilepticus by Activation of the PGC-1α Signaling Pathway.

International journal of molecular sciences

Chuang YC,Chen SD,Hsu CY,Chen SF,Chen NC,Jou SB

35-8100 was used in Western Blotting to examine the neuroprotective effect of resveratrol on mitochondrial biogenesis in the hippocampus following experimental status epilepticus.

Mon Feb 25 00:00:00 UTC 2019

Mechanism of super-assembly of respiratory complexes III and IV.

Nature

Cogliati S,Calvo E,Loureiro M,Guaras AM,Nieto-Arellano R,Garcia-Poyatos C,Ezkurdia I,Mercader N,Vázquez J,Enriquez JA

358100 was used in western blot to elucidate how SCAF1 contributes to the formation of the respirasome

Thu Nov 24 00:00:00 UTC 2016

A cyclooxygenase-2-dependent prostaglandin E2 biosynthetic system in the Golgi apparatus.

The Journal of biological chemistry

Yuan C,Smith WL

35-8100 was used in immunocytochemistry and western blot to investigate a prostaglandin E2 biosynthetic system in the Golgi apparatus

Fri Feb 27 00:00:00 UTC 2015

A cyclooxygenase-2-dependent prostaglandin E2 biosynthetic system in the Golgi apparatus.

The Journal of biological chemistry

Yuan C,Smith WL

35-8100 was used in immunocytochemistry and western blot to investigate a prostaglandin E2 biosynthetic system in the Golgi apparatus

Fri Feb 27 00:00:00 UTC 2015

A cyclooxygenase-2-dependent prostaglandin E2 biosynthetic system in the Golgi apparatus.

The Journal of biological chemistry

Yuan C,Smith WL

35-8100 was used in immunocytochemistry and western blot to investigate a prostaglandin E2 biosynthetic system in the Golgi apparatus

Fri Feb 27 00:00:00 UTC 2015

A cyclooxygenase-2-dependent prostaglandin E2 biosynthetic system in the Golgi apparatus.

The Journal of biological chemistry

Yuan C,Smith WL

35-8100 was used in immunocytochemistry and western blot to investigate a prostaglandin E2 biosynthetic system in the Golgi apparatus

Fri Feb 27 00:00:00 UTC 2015

Effect of ginger root on cyclooxygenase-1 and 15-hydroxyprostaglandin dehydrogenase expression in colonic mucosa of humans at normal and increased risk for colorectal cancer.

European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)

Jiang Y,Turgeon DK,Wright BD,Sidahmed E,Ruffin MT,Brenner DE,Sen A,Zick SM

Sun Sep 01 00:00:00 UTC 2013

Modulation of CXCR3 ligand secretion by prostaglandin E2 and cyclooxygenase inhibitors in human breast cancer.

Breast cancer research : BCR

Bronger H,Kraeft S,Schwarz-Boeger U,Cerny C,Stöckel A,Avril S,Kiechle M,Schmitt M

35-8100 was used in western blot to discuss targeting CXCR3 signaling to treat breast cancer

Tue Feb 14 00:00:00 UTC 2012

Roles of E-cadherin and cyclooxygenase enzymes in predicting different survival patterns of optimally cytoreduced serous ovarian cancer patients.

Asian Pacific journal of cancer prevention : APJCP

Taşkin S,Dünder I,Erol E,Taşkin EA,Kiremitçi S,Öztuna D,Sertçelik A

35-8100 was used in immunohistochemistry (paraffin) to study the relationship between cyclooxygenase enzymes and E-cadherin in the prediction of survival in optimally cytoreduced serous ovarian cancer patients.

Tue Jul 02 00:00:00 UTC 2013

Molecular typing of epithelial ovarian carcinomas using inflammatory markers.

Cancer

Ali-Fehmi R,Semaan A,Sethi S,Arabi H,Bandyopadhyay S,Hussein YR,Diamond MP,Saed G,Morris RT,Munkarah AR

35-8100 was used in immunohistochemistry - paraffin section to characterize type I and II ovarian epithelial carcinomas

Sat Jan 15 00:00:00 UTC 2011

The Ras signaling inhibitor LOX-PP interacts with Hsp70 and c-Raf to reduce Erk activation and transformed phenotype of breast cancer cells.

Molecular and cellular biology

Sato S,Trackman PC,Mäki JM,Myllyharju J,Kirsch KH,Sonenshein GE

35-8100 was used in western blot to identify the interaction of LOX-PP with Hsp70 and c-Raf and investigate how this interaction affects Ras-induced MEK signaling.

Fri Jul 01 00:00:00 UTC 2011

Amino acid-based ionic liquids: using XPS to probe the electronic environment via binding energies.

Physical chemistry chemical physics : PCCP

Hurisso BB,Lovelock KR,Licence P

Fri Oct 21 00:00:00 UTC 2011

Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure.

PloS one

Lundström SL,Levänen B,Nording M,Klepczynska-Nyström A,Sköld M,Haeggström JZ,Grunewald J,Svartengren M,Hammock BD,Larsson BM,Eklund A,Wheelock ÅM,Wheelock CE

35-8100 was used in immunocytochemistry to discuss pollutants as contributors to asthma.

Thu Dec 22 00:00:00 UTC 2011

Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure.

PloS one

Lundström SL,Levänen B,Nording M,Klepczynska-Nyström A,Sköld M,Haeggström JZ,Grunewald J,Svartengren M,Hammock BD,Larsson BM,Eklund A,Wheelock ÅM,Wheelock CE

35-8100 was used in immunocytochemistry to discuss pollutants as contributors to asthma.

Thu Dec 22 00:00:00 UTC 2011

Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis.

Oral diseases

Ketabchi S,Massi D,Ficarra G,Rubino I,Franchi A,Paglierani M,Simoni A,Capodiferro S,Favia G,Maiorano E,Tarantini F,Cirino G,Santucci M

35-8100 was used in immunohistochemistry - paraffin section to determine the expression of PAR-1, PAR-2, MMP-2, MMP-9, COX-1, and COX-2 in patients with orofacial granulomatosis

Sun Jul 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: COX-1; cyclooxygenase 3; cyclooxygenase precursor (EC 1.14.99.1); Cyclooxygenase-1; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1; P PGG/HS; PGH synthase 1; PHS 1; prostaglandin endoperoxide synthase; Prostaglandin G/H synthase 1; prostaglandin G/H synthase and cyclooxygenase; prostaglandin G/H synthase precursor (EC 1.14.99.1); Prostaglandin H2 synthase 1; Prostaglandin-endoperoxide synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); unnamed protein product

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Gene Aliases: COI; COX-1; Cox-3; COX1; COX3; COXI; MT-CO1; MTCO1; PCOX1; PES-1; PGG/HS; PGHS-1; PGHS1; PHS 1; PHS1; PTGHS; PTGS1

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UniProt ID: (Human) P23219, (Sheep) P05979, (Mouse) P22437

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Entrez Gene ID: (Human) 5742, (Sheep) 443551, (Rat) 24693, (Mouse) 19224

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Function(s)

peroxidase activity prostaglandin-endoperoxide synthase activity lipid binding heme binding metal ion binding dioxygenase activity oxidoreductase activity

Process(es)

prostaglandin biosynthetic process lipid metabolic process xenobiotic metabolic process inflammatory response response to oxidative stress aging learning memory regulation of blood pressure response to organonitrogen compound negative regulation of norepinephrine secretion sensory perception of pain cyclooxygenase pathway negative regulation of epinephrine secretion maintenance of blood-brain barrier regulation of cell proliferation positive regulation of vasoconstriction positive regulation of smooth muscle contraction response to corticosterone oxidation-reduction process response to fatty acid cellular oxidant detoxification prostaglandin metabolic process fatty acid metabolic process fatty acid biosynthetic process keratinocyte differentiation

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COX1 Monoclonal Antibody (COX 111)

COX1 Antibody (35-8100) in IHC

Product Details

35-8100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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