Invitrogen

COX2 Monoclonal Antibody (COX 229), PE-Cyanine7, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

25-9725-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.125 µg
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

COX 229

Immunogen

Synthetic peptide from human COX2

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802269

Product Specific Information

Description: This COX 229 monoclonal antibody recognizes human COX2 also known as PTGS2. This antibody has been reported to cross-react with the mouse and rat COX2. For optimal performance, we recommend using this antibody with the Foxp3 / Transcription Factor Staining Buffer Set and protocol.

Applications Reported: This COX 229 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This COX 229 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

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Gene Aliases: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2; PTGS2

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UniProt ID: (Human) P35354

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Entrez Gene ID: (Human) 5743

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Function(s)
peroxidase activity prostaglandin-endoperoxide synthase activity protein binding lipid binding enzyme binding heme binding protein homodimerization activity metal ion binding arachidonate 15-lipoxygenase activity
Process(es)
prostaglandin biosynthetic process angiogenesis prostaglandin metabolic process nicotinamide metabolic process movement of cell or subcellular component inflammatory response response to oxidative stress embryo implantation learning memory regulation of blood pressure negative regulation of cell proliferation response to fructose response to manganese ion response to lithium ion positive regulation of vascular endothelial growth factor production sensory perception of pain cyclooxygenase pathway lipoxygenase pathway bone mineralization ovulation positive regulation of prostaglandin biosynthetic process positive regulation of fever generation positive regulation of synaptic plasticity negative regulation of synaptic transmission, dopaminergic response to estradiol response to lipopolysaccharide response to vitamin D response to tumor necrosis factor cellular response to UV maintenance of blood-brain barrier positive regulation of NF-kappaB import into nucleus response to drug hair cycle positive regulation of apoptotic process positive regulation of nitric oxide biosynthetic process negative regulation of cell cycle positive regulation of vasoconstriction negative regulation of smooth muscle contraction positive regulation of smooth muscle contraction decidualization positive regulation of smooth muscle cell proliferation regulation of inflammatory response brown fat cell differentiation response to glucocorticoid negative regulation of calcium ion transport positive regulation of synaptic transmission, glutamatergic oxidation-reduction process response to fatty acid cellular response to mechanical stimulus cellular response to ATP cellular response to hypoxia cellular response to fluid shear stress positive regulation of transforming growth factor beta production positive regulation of cell migration involved in sprouting angiogenesis positive regulation of fibroblast growth factor production positive regulation of brown fat cell differentiation positive regulation of platelet-derived growth factor production cellular oxidant detoxification
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