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Invitrogen
COX4 Polyclonal Antibody
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FIGURE: 1 / 15
COX4 Antibody (PA5-95519) in ICC/IF
Immunocytochemistry analysis of COX IV using anti-COX IV antibody (Product # PA5-95519). COX IV was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-COX IV antibody (Product # PA5-95519) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visual... View More
Product Details
PA5-95519
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human COX IV recombinant protein (Position: Q59-K169).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807321
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human CACO-2 whole cell, human SiHa whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: mouse small intestine tissue, rat lung tissue, rat small intestine tissue, rat liver tissue, human placenta tissue, human thyroid cancer tissue, human intestinal cancer tissue, mouse kidney tissue. ICC/IF: U20S cell. Flow: U937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4 (COX4I1) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cox iv; COX IV-1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; cytochrome c oxidase, subunit 4a; cytochrome c oxidase, subunit IV; cytochrome c oxidase, subunit IVa; FLJ23483; MGC72016
Gene Aliases: AL024441; COX; COX IV-1; COX4; COX4-1; Cox4a; COX4I1; COXIV; COXIV-1; IV-1
UniProt ID: (Human) P13073, (Rat) P10888, (Mouse) P19783
Entrez Gene ID: (Human) 1327, (Rat) 29445, (Mouse) 12857
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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