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Invitrogen
COX5A Monoclonal Antibody (6E9B12D5)
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FIGURE: 1 / 6
COX5A Antibody (459120) in ICC/IF
Immunofluorescent analysis of Complex IV in MRC5 cells using a Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized, and then labeled with Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. An AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody at a concentration of 1 µg/mL was used for detection.
Product Details
459120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
6E9B12D5
Immunogen
Bovine COX5A
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10376328
Product Specific Information
Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells.
Target Information
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytochrome c oxidase polypeptide Va; cytochrome c oxidase polypeptide, mitochondrial; Cytochrome c oxidase subunit 5A, mitochondrial; cytochrome c oxidase subunit Va; mitochondrial cytochrome c oxidase subunit 5a; mitochondrial cytochrome c oxidase subunit Va
Gene Aliases: AA959768; BOS_20221; CcOX; COX; COX-VA; COX5A; VA
UniProt ID: (Human) P20674, (Bovine) P00426, (Rat) P11240, (Mouse) P12787
Entrez Gene ID: (Human) 9377, (Bovine) 444878, (Rat) 252934, (Mouse) 12858
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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