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Invitrogen
COX5B Polyclonal Antibody
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COX5B Antibody (PA5-116487) in IHC (P)
Immunohistochemical analysis of COX5B in a paraffin-embedded human small intestine tissue using a COX5B polyclonal antibody (Product #PA5-116487). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:200... View More
Product Details
PA5-116487
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within Human COX5B aa 50-99
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901118
Product Specific Information
Positive controls: 293 cell lysates, human liver tissue, human liver carcinoma tissue, human kidney tissue, human small intestine tissue, HepG2.
Target Information
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytochrome c oxidase polypeptide Vb; cytochrome c oxidase polypeptide VB, mitochondrial; Cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb
Gene Aliases: COX5B; COXVB
UniProt ID: (Human) P10606
Entrez Gene ID: (Human) 1329
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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