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Invitrogen
COX5B Monoclonal Antibody (16H12H9)
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FIGURE: 1 / 5
COX5B Antibody (459110) in WB
Western blot analysis of COX5B in heart mitochondria extracts using a COX5B Monoclonal antibody (Product # 459110) at a concentration of 1 µg/mL. Lane 1 : Human heart mitochondria at 5 µg, Lane 2 : Bovine heart mitochondria at 1 µg, Lane 3 : Rat heart mitochondria at 10 µg, Lane 4 : Mouse heart mitochondria at 10 µg. Predicted band size: 14 kDa.
Product Details
459110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
16H12H9
Immunogen
Bovine Complex IV (native holoenzyme protein, purified from heart)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10376329
Product Specific Information
Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart
Target Information
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytochrome c oxidase polypeptide Vb; cytochrome c oxidase polypeptide VB, mitochondrial; Cytochrome c oxidase polypeptide VIa; Cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; Cytochrome c oxidase subunit VIA*
Gene Aliases: BOS_11259; COX5B; COXVB
UniProt ID: (Human) P10606, (Bovine) P00428, (Rat) P12075
Entrez Gene ID: (Human) 1329, (Bovine) 287012, (Rat) 94194, (Mouse) 12859
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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