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Invitrogen
CPO Polyclonal Antibody
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FIGURE: 1 / 3
CPO Antibody (PA5-143905) in WB
Western blot analysis of CPO using CPO antibody (Product # PA5-143905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates; Lane 2: human COLO-320 whole cell lysates; Lane 3: human SW620 whole cell lysates; Lane 4: human AGS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blo... View More
Product Details
PA5-143905
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CPO recombinant protein (Position: D34-V280).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075119
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Caco-2 whole cell, human COLO-320 whole cell, human SW620 whole cell, human AGS whole cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Probable carboxypeptidase which may cleave proteins with C-terminal acidic residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carboxypeptidase O; CPO; metallocarboxypeptidase C; metallocarboxypeptidase O
Gene Aliases: CPO
UniProt ID: (Human) Q8IVL8
Entrez Gene ID: (Human) 130749
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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