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Invitrogen
CPSF3 Recombinant Rabbit Monoclonal Antibody (4S4P2)
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FIGURE: 1 / 2
CPSF3 Antibody (MA5-42768) in WB
Western blot analysis of CPSF3 in extracts of various cell lines. Samples were incubated with CPSF3 Monoclonal antibody (Product # MA5-42768) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
MA5-42768
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4S4P2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 585-684 of human CPSF3 (Q9UKF6)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911909
Product Specific Information
Positive test controls include: HeLa, 293T, SH-SY5Y, Mouse liver, Mouse kidney, Mouse uterus, Rat spleen. The target is usually found in the following locations: Nucleus.
Immunogen sequence: LEVQSNPKIR KGAVQKVSKK LEMHVYSKRL EIMLQDIFGE DCVSVKDDSI LSVTVDGKTA NLNLETRTVE CEEGSEDDES LREMVELAAQ RLYEALTPVH
Target Information
Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al., 2006).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cleavage and polyadenylation specific factor 3, 73kDa; cleavage and polyadenylation specificity factor 3; Cleavage and polyadenylation specificity factor 73 kDa subunit; Cleavage and polyadenylation specificity factor subunit 3; CPSF; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73; YSH1
Gene Aliases: CPSF-73; CPSF3; CPSF73
UniProt ID: (Human) Q9UKF6, (Mouse) Q9QXK7
Entrez Gene ID: (Human) 51692, (Mouse) 54451, (Rat) 298916
mRNA processing
mRNA 3'-end processing by stem-loop binding and cleavage
RNA phosphodiester bond hydrolysis, endonucleolytic
mRNA splicing, via spliceosome
termination of RNA polymerase II transcription
mRNA polyadenylation
mRNA cleavage
mRNA export from nucleus
mRNA 3'-end processing
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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