Proteintech

CPSF4 Polyclonal Antibody

CPSF4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

CPSF4 Antibody (15023-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using CPSF4 antibody 15023-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

CPSF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CPSF4 Antibody in Immunoprecipitation (IP)

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Product Details

15023-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CPSF4 Fusion Protein Ag7103 (1-244 aa encoded by BC003101)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MQEIIASVD HIKFDLEIAV EQQLGAQPLP FPGMDKSGAA VCEFFLKAAC GKGGMCPFRH ISGEKTVVCK HWLRGLCKKG DQCEFLHEYD MTKMPECYFY SKFGECSNKE CPFLHIDPES KIKDCPWYDR GFCKHGPLCR HRHTRRVICV NYLVGFCPEG PSCKFMHPRF ELPMGTTEQP PLPQQTQPPA KQRTPQVIGV MQSQNSSAGN RGPRPLEQVT CYKCGEKGHY ANRCTKGHLA FLSGQ (1-244 aa encoded by BC003101)

Target Information

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors.

International journal of biological macromolecules

Teng Z,Hao L,Yang R,Song J,Wang Z,Jiao Y,Fang J,Zheng S,Ma Z,Chen X,Liu S,Cheng Y

Tue May 31 00:00:00 UTC 2022

Nuclear m⁶ A reader YTHDC1 suppresses proximal alternative polyadenylation sites by interfering with the 3' processing machinery.

EMBO reports

Chen L,Fu Y,Hu Z,Deng K,Song Z,Liu S,Li M,Ou X,Wu R,Liu M,Li R,Gao S,Cheng L,Chen S,Xu A

Mon Nov 07 00:00:00 UTC 2022

CPSF4 promotes tumor-initiating phenotype by enhancing VEGF/NRP2/TAZ signaling in lung cancer.

Medical oncology (Northwood, London, England)

Song Y,Sun K,Gong L,Shi L,Qin T,Wang S,Deng W,Chen W,Zheng F,Li G

Mon Dec 26 00:00:00 UTC 2022

CPSF4 promotes tumor-initiating phenotype by enhancing VEGF/NRP2/TAZ signaling in lung cancer.

Medical oncology (Northwood, London, England)

Song Y,Sun K,Gong L,Shi L,Qin T,Wang S,Deng W,Chen W,Zheng F,Li G

Mon Dec 26 00:00:00 UTC 2022

MiR-4458 inhibits breast cancer cell growth, migration, and invasiveness by targeting CPSF4.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Wu J,Miao J,Ding Y,Zhang Y,Huang X,Zhou X,Tang R

Sun Dec 01 00:00:00 UTC 2019

MiR-4458 inhibits breast cancer cell growth, migration, and invasiveness by targeting CPSF4.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Wu J,Miao J,Ding Y,Zhang Y,Huang X,Zhou X,Tang R

Sun Dec 01 00:00:00 UTC 2019

The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis.

Scientific reports

Ashraf S,Radhi M,Gowler P,Burston JJ,Gandhi RD,Thorn GJ,Piccinini AM,Walsh DA,Chapman V,de Moor CH

Mon Mar 18 00:00:00 UTC 2019

The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis.

Scientific reports

Ashraf S,Radhi M,Gowler P,Burston JJ,Gandhi RD,Thorn GJ,Piccinini AM,Walsh DA,Chapman V,de Moor CH

Mon Mar 18 00:00:00 UTC 2019

Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.

Biochimica et biophysica acta. Molecular cell research

Yang Q,Fan W,Zheng Z,Lin S,Liu C,Wang R,Li W,Zuo Y,Sun Y,Hu S,Chen M,Guo P,Pan J,Tian C,Zhu T,Diao C,Hao J,Yu W,Li L,Li Y,Guo W,Deng W,Wu X

Tue Oct 01 00:00:00 UTC 2019

Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.

Biochimica et biophysica acta. Molecular cell research

Yang Q,Fan W,Zheng Z,Lin S,Liu C,Wang R,Li W,Zuo Y,Sun Y,Hu S,Chen M,Guo P,Pan J,Tian C,Zhu T,Diao C,Hao J,Yu W,Li L,Li Y,Guo W,Deng W,Wu X

Tue Oct 01 00:00:00 UTC 2019

Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.

Cancer letters