Invitrogen

CRABP1 Monoclonal Antibody (C-1)

CRABP1 Antibody in Immunocytochemistry (ICC/IF)

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CRABP1 Antibody (MA3-813) in ICC/IF

Immunofluorescent analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) (Product # MA3-813) shows staining in NIH-3T3 Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI (Product # MA3-813) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product... View More

CRABP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CRABP1 Antibody in Flow Cytometry (Flow)

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Product Details

MA3-813

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:250

Immunocytochemistry (ICC/IF)

1:100-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Chicken, Cat, Human, Mouse, Non-human primate, Rabbit, Reptile, Rat

Published species

Avian, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

C-1

Immunogen

Oxidized CRABPI from bovine retina.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography, Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2085320

Product Specific Information

MA3-813 detects cellular retinoic acid binding protein I (CRABPI) from human, mouse, monkey, feline, bovine, rabbit, rat, turtle and chicken tissues. This antibody does not react with chameleon, goldfish and frog. This antibody does not cross-react with cellular retinol binding protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein.

MA3-813 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 16 kDa protein representing CRABPI from rat retinal supernatant. Immunohistochemical staining of CRABPI in rat retina with MA3-813 results in its localization to amacrine somata and laminae in the inner plexiform layer.

The MA3-813 antigen is oxidized CRABPI from bovine retina.

Target Information

This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting Cellular Retinoic Acid Binding Protein 1 with Retinoic Acid-like Compounds to Mitigate Motor Neuron Degeneration.

International journal of molecular sciences

Nhieu J,Milbauer L,Lerdall T,Najjar F,Wei CW,Ishida R,Ma Y,Kagechika H,Wei LN

MA3-813 was used in Western Blotting to provide insight into the potential of signaling pathway-selective, CRABP1-binding, atRA-like ligands in mitigating MN degenerative diseases.

Sat Mar 04 00:00:00 UTC 2023

Targeting Cellular Retinoic Acid Binding Protein 1 with Retinoic Acid-like Compounds to Mitigate Motor Neuron Degeneration.

International journal of molecular sciences

Nhieu J,Milbauer L,Lerdall T,Najjar F,Wei CW,Ishida R,Ma Y,Kagechika H,Wei LN

MA3-813 was used in Western Blotting to provide insight into the potential of signaling pathway-selective, CRABP1-binding, atRA-like ligands in mitigating MN degenerative diseases.

Sat Mar 04 00:00:00 UTC 2023

Targeting Cellular Retinoic Acid Binding Protein 1 with Retinoic Acid-like Compounds to Mitigate Motor Neuron Degeneration.

International journal of molecular sciences

Nhieu J,Milbauer L,Lerdall T,Najjar F,Wei CW,Ishida R,Ma Y,Kagechika H,Wei LN

MA3-813 was used in Western Blotting to provide insight into the potential of signaling pathway-selective, CRABP1-binding, atRA-like ligands in mitigating MN degenerative diseases.

Sat Mar 04 00:00:00 UTC 2023

Targeting Cellular Retinoic Acid Binding Protein 1 with Retinoic Acid-like Compounds to Mitigate Motor Neuron Degeneration.

International journal of molecular sciences

Nhieu J,Milbauer L,Lerdall T,Najjar F,Wei CW,Ishida R,Ma Y,Kagechika H,Wei LN

MA3-813 was used in Western Blotting to provide insight into the potential of signaling pathway-selective, CRABP1-binding, atRA-like ligands in mitigating MN degenerative diseases.

Sat Mar 04 00:00:00 UTC 2023

Myofibroblast transcriptome indicates SFRP2^hi fibroblast progenitors in systemic sclerosis skin.

Nature communications

Tabib T,Huang M,Morse N,Papazoglou A,Behera R,Jia M,Bulik M,Monier DE,Benos PV,Chen W,Domsic R,Lafyatis R

MA3-813 was used in Immunohistochemistry to define transcriptomic changes in different fibroblast populations present in systemic sclerosis.

Mon Jul 19 00:00:00 UTC 2021

Proteome analysis of the effects of all-trans retinoic acid on human germ cell tumor cell lines.

Journal of proteomics

Honecker F,Rohlfing T,Harder S,Braig M,Gillis AJ,Glaesener S,Barett C,Bokemeyer C,Buck F,Brümmendorf TH,Looijenga LH,Balabanov S

MA3-813 was used in western blot to study the effects all-trans retinoic on human germ cell tumor cells using proteomics

Thu Jan 16 00:00:00 UTC 2014

Isolation and properties of an in vitro human outer blood-retinal barrier model.

Methods in molecular biology (Clifton, N.J.)

Hamilton RD,Leach L

MA3-813 was used in immunocytochemistry to characterize an invitro human outer blood-retinal barrier model

Wed Jun 01 00:00:00 UTC 2011

Retinoic acid orchestrates fibroblast growth factor signalling to drive embryonic stem cell differentiation.

Development (Cambridge, England)

Stavridis MP,Collins BJ,Storey KG

MA3-813 was used in western blot to investigate the effect of retinoic acid on embryonic stem cell differentiation

Mon Mar 01 00:00:00 UTC 2010

LIF removal increases CRABPI and CRABPII transcripts in embryonic stem cells cultured in retinol or 4-oxoretinol.

Molecular and cellular endocrinology

Lane MA,Xu J,Wilen EW,Sylvester R,Derguini F,Gudas LJ

MA3-813 was used in western blot to study the effect of leukemia inhibitory factor on CRABPI and CRABPII transcription in embryonic stem cells

Wed Jan 02 00:00:00 UTC 2008

Establishment of a human in vitro model of the outer blood-retinal barrier.

Journal of anatomy

Hamilton RD,Foss AJ,Leach L

MA3-813 was used in immunohistochemistry to establish a human in vitro model of the outer blood-retinal barrier

Sat Dec 01 00:00:00 UTC 2007

In cerebrospinal fluid ER chaperones ERp57 and calreticulin bind beta-amyloid.

Biochemical and biophysical research communications

Erickson RR,Dunning LM,Olson DA,Cohen SJ,Davis AT,Wood WG,Kratzke RA,Holtzman JL

MA3-813 was used in western blot to elucidate ER chaperones calreticulin and ERp57 bind beta-amyloid in cerebrospinal fluid

Fri Jun 24 00:00:00 UTC 2005

Differential gene expression in ovarian carcinoma: identification of potential biomarkers.

The American journal of pathology

Hibbs K,Skubitz KM,Pambuccian SE,Casey RC,Burleson KM,Oegema TR,Thiele JJ,Grindle SM,Bliss RL,Skubitz AP

MA3-813 was used in immunohistochemistry to investigate the diagnostic, therapeutic, and prognostic biomarkers in ovarian carcinoma

Sun Aug 01 00:00:00 UTC 2004

SFRP1 modulates retina cell differentiation through a beta-catenin-independent mechanism.

Journal of cell science

Esteve P,Trousse F,Rodríguez J,Bovolenta P

MA3-813 was used in immunocytochemistry to investigate the mechanism for the effect of SFRP1 on retina cell differentiation

Sun Jun 15 00:00:00 UTC 2003

Retinoic acid promotes differentiation of photoreceptors in vitro.

Development (Cambridge, England)

Kelley MW,Turner JK,Reh TA

MA3-813 was used in immunohistochemistry to define the involvement of retinoic acid in the development of the nervous system

Mon Aug 01 00:00:00 UTC 1994

The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture.

Development (Cambridge, England)

Kelley MW,Xu XM,Wagner MA,Warchol ME,Corwin JT

MA3-813 was used in western blot to study the involvement of retinoic acid in the development of organ of Corti

Wed Dec 01 00:00:00 UTC 1993

Immunolocalization of cellular retinol-, retinaldehyde- and retinoic acid-binding proteins in rat retina during pre- and postnatal development.

Journal of neurocytology

De Leeuw AM,Gaur VP,Saari JC,Milam AH

MA3-813 was used in immunocytochemistry to investigate the cellular distribution of CRBP, CRALBP and CRABP in rat retina

Sun Apr 01 00:00:00 UTC 1990

Bioinformatics

Protein Aliases: cellular retinoic acid binding protein 1; cellular retinoic acid binding protein I; cellular retinoic acid binding protein I (CRABP-I) homologue; Cellular retinoic acid-binding protein 1; Cellular retinoic acid-binding protein I; CRABP-I

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Gene Aliases: AI326249; BOS_20217; cCRABP-I; CRABP; Crabp-1; CRABP-I; CRABP1; CRABPI; Rbp-5; RBP5

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UniProt ID: (Human) P29762, (Bovine) P62964, (Chicken) P40220, (Rat) P62966, (Mouse) P62965

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Entrez Gene ID: (Human) 1381, (Bovine) 282201, (Chicken) 374211, (Rat) 25061, (Mouse) 12903, (Rabbit) 100338607

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Function(s)

retinoic acid binding transporter activity retinoid binding protein binding retinal binding retinol binding lipid binding

Process(es)

transport signal transduction multicellular organism development retinoic acid catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CRABP1 Monoclonal Antibody (C-1)

CRABP1 Antibody (MA3-813) in ICC/IF

Product Details

MA3-813

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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