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CRACR2A (EFCAB4B) Antibody (ACC-324-200UL) in WB
Western blot analysis of human T cell leukemia (Jurkat) (lanes 1 and 3) and rat stomach (lanes 2 and 4) lysates: - 1,2. Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324), (1:400).3,4. Anti-CRACR2A (EFCAB4B) Antibody , preincubated with CRACR2A/EFCAB4B Blocking Peptide (#BLP-CC324).
Product Details
ACC-324-200UL
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A, Intracellular
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
CRACR2A is a Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. CRACR2A acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. Further, CRACR2A regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, CRACR2A dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ca2+ release-activated Ca2+ (CRAC) channel regulator 2A; calcium release activated channel regulator 2A; Calcium release-activated calcium channel regulator 2A; Calcium release-activated channel regulator 2A; CRAC channel regulator 2A; CRAC regulator 2A; EF-hand calcium binding domain 4B; EF-hand calcium-binding domain-containing protein 4B; Ras-related protein Rab-46
Gene Aliases: CRACR2A; EFCAB4B; RAB46; RGD1563719
UniProt ID: (Human) Q9BSW2
Entrez Gene ID: (Human) 84766, (Rat) 500324
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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