Invitrogen

CREB Monoclonal Antibody (LB9)

CREB Antibody in Immunocytochemistry (ICC/IF)

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CREB Antibody (35-0900) in ICC/IF

Immunofluorescent analysis of CREB was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with CREB Mouse Monoclonal Antibody (Product # 35-0900) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at roo... View More

CREB Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

Product Image Gallery

CREB Antibody — IP-MS enrichment of CREB1 (LFQ intensity): CREB1 was enriched 136-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and CREB1 antibody (Product # 35-0900). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

35-0900

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-3 µg/mL

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LB9

Immunogen

Recombinant human CREB-A protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

AB_2533194

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mTOR Signaling Pathway Regulates the Release of Proinflammatory Molecule CCL5 Implicated in the Pathogenesis of Autism Spectrum Disorder.

Frontiers in immunology

Wang B,Qin Y,Wu Q,Li X,Xie D,Zhao Z,Duan S

35-0900 was used in Simple Western - Size to show that the phosphorylated CREB can be induced to suppress CCL5 expression by outcompeting NF-κB in binding to CREB-binding protein (CREBBP) once the mTOR signaling pathway is inhibited.

Mon Apr 18 00:00:00 UTC 2022

mTOR Signaling Pathway Regulates the Release of Proinflammatory Molecule CCL5 Implicated in the Pathogenesis of Autism Spectrum Disorder.

Frontiers in immunology

Wang B,Qin Y,Wu Q,Li X,Xie D,Zhao Z,Duan S

Mon Apr 18 00:00:00 UTC 2022

Cannabinoid receptor 1 signaling in hepatocytes and stellate cells does not contribute to NAFLD.

The Journal of clinical investigation

Wang S,Zhu Q,Liang G,Franks T,Boucher M,Bence KK,Lu M,Castorena CM,Zhao S,Elmquist JK,Scherer PE,Horton JD

35-0900 was used in Western Blot to provide evidence against the hypothesis that hepatocyte or stellate cell CB-1 signaling has a direct role in the development of nonalcoholic fatty liver disease or liver fibrosis.

Mon Nov 15 00:00:00 UTC 2021

Central administration of afzelin extracted from Ribes fasciculatum improves cognitive and memory function in a mouse model of dementia.

Scientific reports

Oh SY,Jang MJ,Choi YH,Hwang H,Rhim H,Lee B,Choi CW,Kim MS

Published figure using CREB monoclonal antibody (Product # 35-0900) in Immunohistochemistry (PFA fixed)

Wed Apr 28 00:00:00 UTC 2021

Central administration of afzelin extracted from Ribes fasciculatum improves cognitive and memory function in a mouse model of dementia.

Scientific reports

Oh SY,Jang MJ,Choi YH,Hwang H,Rhim H,Lee B,Choi CW,Kim MS

Published figure using CREB monoclonal antibody (Product # 35-0900) in Immunohistochemistry (PFA fixed)

Wed Apr 28 00:00:00 UTC 2021

Central administration of afzelin extracted from Ribes fasciculatum improves cognitive and memory function in a mouse model of dementia.

Scientific reports

Oh SY,Jang MJ,Choi YH,Hwang H,Rhim H,Lee B,Choi CW,Kim MS

Published figure using CREB monoclonal antibody (Product # 35-0900) in Immunohistochemistry (PFA fixed)

Wed Apr 28 00:00:00 UTC 2021

Acetyl CoA Carboxylase Inhibition Reduces Hepatic Steatosis but Elevates Plasma Triglycerides in Mice and Humans: A Bedside to Bench Investigation.

Cell metabolism

Kim CW,Addy C,Kusunoki J,Anderson NN,Deja S,Fu X,Burgess SC,Li C,Ruddy M,Chakravarthy M,Previs S,Milstein S,Fitzgerald K,Kelley DE,Horton JD

35-0900 was used in Western Blot to determine if reducing lipogenesis prevents hepatic steatosis in humans, as in rodents with insulin resistance.

Tue Aug 01 00:00:00 UTC 2017

Menin and PRMT5 suppress GLP1 receptor transcript and PKA-mediated phosphorylation of FOXO1 and CREB.

American journal of physiology. Endocrinology and metabolism

Muhammad AB,Xing B,Liu C,Naji A,Ma X,Simmons RA,Hua X

35-0900 was used in Immunoprecipitation and western blot to investigate whether menin crosstalks with glucagon-like-peptide-1 signalling.

Tue Aug 01 00:00:00 UTC 2017

Menin and PRMT5 suppress GLP1 receptor transcript and PKA-mediated phosphorylation of FOXO1 and CREB.

American journal of physiology. Endocrinology and metabolism

Muhammad AB,Xing B,Liu C,Naji A,Ma X,Simmons RA,Hua X

35-0900 was used in Immunoprecipitation and western blot to investigate whether menin crosstalks with glucagon-like-peptide-1 signalling.

Tue Aug 01 00:00:00 UTC 2017

Long-term treatment with standardized extract of Ginkgo biloba L. enhances the conditioned suppression of licking in rats by the modulation of neuronal and glial cell function in the dorsal hippocampus and central amygdala.

Neuroscience

Oliveira DR,Sanada PF,Filho AC,Conceição GM,Cerutti JM,Cerutti SM

35-0900 was used in immunohistochemistry to assess the effects of chronic treatment with Ginkgo biloba extract on the conditioned emotional response.

Wed Apr 03 00:00:00 UTC 2013

Neuromodulatory property of standardized extract Ginkgo biloba L. (EGb 761) on memory: behavioral and molecular evidence.

Brain research

Oliveira DR,Sanada PF,Saragossa Filho AC,Innocenti LR,Oler G,Cerutti JM,Cerutti SM

35-0900 was used in immunohistochemistry to assess the effects of acute or subacute treatments with Ginkgo biloba leaf extract on rats submitted to a conditioned emotional response

Thu May 07 00:00:00 UTC 2009

Inactivation of CREB mediated gene transcription by HDAC8 bound protein phosphatase.

Biochemical and biophysical research communications

Gao J,Siddoway B,Huang Q,Xia H

Fri Jan 30 00:00:00 UTC 2009

Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins.

The Journal of clinical investigation

Engelking LJ,Liang G,Hammer RE,Takaishi K,Kuriyama H,Evers BM,Li WP,Horton JD,Goldstein JL,Brown MS

35-0900 was used in western blot to uses knockout mice to study the feedback inhibition of cholesterol synthesis in vivo

Thu Sep 01 00:00:00 UTC 2005

Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins.

The Journal of clinical investigation

Engelking LJ,Liang G,Hammer RE,Takaishi K,Kuriyama H,Evers BM,Li WP,Horton JD,Goldstein JL,Brown MS

35-0900 was used in western blot to uses knockout mice to study the feedback inhibition of cholesterol synthesis in vivo

Thu Sep 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: active transcription factor CREB; cAMP response element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein

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Gene Aliases: 2310001E10Rik; 3526402H21Rik; AV083133; CREB; CREB-1; CREB1

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UniProt ID: (Human) P16220, (Mouse) Q01147, (Rat) P15337

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Entrez Gene ID: (Human) 1385, (Mouse) 12912, (Rat) 81646

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor activity protein binding enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding DNA binding double-stranded DNA binding transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CREB Monoclonal Antibody (LB9)

CREB Antibody (35-0900) in ICC/IF

Product Details

35-0900

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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