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FIGURE: 1 / 1
CRM1 Antibody (PA5-87417) in WB
Western blot analysis of CRM1 in extracts of various cell lines. Samples were incubated with CRM1 Polyclonal antibody (Product # PA5-87417) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-87417
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 964-1064 of human CRM1/CRM1/XPO1 (NP_0033911)
Conjugate
Form
Liquid
Concentration
1.85 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804132
Product Specific Information
Positive Samples: U-87MG, B-cell, Mouse liver; Cellular Location: Cajal body, Cytoplasm, Nucleus, nucleolus, nucleoplasm
Immunogen sequence: PGNPVNNQIF LQEYVANLLK SAFPHLQDAQ VKLFVTGLFS LNQDIPAFKE HLRDFLVQIK EFAGEDTSDL FLEEREIALR QADEEKHKRQ MSVPGIFNPH E
Target Information
Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chromosome region maintenance 1 homolog; Chromosome region maintenance 1 protein homolog; Exp1; exportin 1 (CRM1 homolog, yeast); Exportin-1; exportin-1 (required for chromosome region maintenance)
Gene Aliases: AA420417; CRM1; emb; Exp1; XPO1
UniProt ID: (Human) O14980, (Mouse) Q6P5F9
Entrez Gene ID: (Human) 7514, (Mouse) 103573
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
negative regulation of transcription from RNA polymerase II promoter
protein export from nucleus
transport
intracellular protein transport
regulation of centrosome duplication
protein transport
protein localization to nucleus
regulation of protein catabolic process
regulation of protein export from nucleus
mRNA transport
sister chromatid cohesion
viral process
response to drug
regulation of mRNA stability
intracellular transport of virus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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