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Invitrogen
CRM1 Recombinant Rabbit Monoclonal Antibody (JB35-22)
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FIGURE: 1 / 12
CRM1 Antibody (MA5-41253) in ICC/IF
Immunofluorescence analysis of XPO1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CRM1 Recombinant Rabbit Monoclonal Antibody (JB35-22) (Product # MA5-41253) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More
Product Details
MA5-41253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB35-22
Immunogen
Recombinant protein within C-terminal Human CRM1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899006
Target Information
Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chromosome region maintenance 1 homolog; Chromosome region maintenance 1 protein homolog; Exp1; exportin 1 (CRM1 homolog, yeast); exportin 1 (CRM1, yeast, homolog); exportin 1, CRM1 homolog; Exportin-1; exportin-1 (required for chromosome region maintenance)
Gene Aliases: AA420417; CRM1; emb; Exp1; XPO1
UniProt ID: (Human) O14980, (Rat) Q80U96, (Mouse) Q6P5F9
Entrez Gene ID: (Human) 7514, (Rat) 85252, (Mouse) 103573
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
negative regulation of transcription from RNA polymerase II promoter
protein export from nucleus
regulation of centrosome duplication
protein localization to nucleus
regulation of protein catabolic process
response to drug
regulation of protein export from nucleus
mRNA transport
transport
intracellular protein transport
protein transport
sister chromatid cohesion
viral process
regulation of mRNA stability
intracellular transport of virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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