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Alomone Labs, LTD
CRTH2/GPR44 (extracellular) Polyclonal Antibody
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CRTH2/GPR44 (extracellular) Antibody (APR-062-200UL) in IHC (F)
Expression ofDP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200), followed by goat- Anti-rabbit-AlexaFluor-488. A. DP2 receptor staining (green) in rat brain sections is detected in neuron-like profiles (arrows) in the medial septum/diagona... View More
Product Details
APR-062-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2, 3rd extracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. At least two different isoforms of CRTH2 are known to exist. Single nucleotide polymorphisms in the 3' UTR of the CRTH2 gene have been associated with asthma susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G protein-coupled receptor 44; G-protein coupled receptor 44; MGC130436; PGD2 receptor; Prostaglandin D2 receptor 2; prostaglandin D2 receptor CRTH2
Gene Aliases: Crth2; Gpr44; Grp45; Ptgdr2
UniProt ID: (Mouse) Q9Z2J6, (Rat) Q6XKD3
Entrez Gene ID: (Mouse) 14764, (Rat) 309212
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
calcium-mediated signaling
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of male germ cell proliferation
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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