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Invitrogen
CRYAB Polyclonal Antibody
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CRYAB Antibody (PA1-16950) in WB
Western blot was performed using Anti-CRYAB Polyclonal Antibody (Product # PA1-16950) and a 20 kDa band corresponding to CRYAB was observed only in Mouse Eye and Rat Eye and not in any other tissues. Tissue extracts (30 µg lysate) of Mouse Eye (Lane 1), Rat Eye (Lane 2), Mouse Heart (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Lung (Lane 5) and Mouse Liver (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001... View More
Product Details
PA1-16950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide between amino acids 70-140 of the human Crystallin AB protein.
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2084434
Product Specific Information
This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa.
There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex.
CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.Suggested positive control: human and mouse skeletal muscle lysate, human skeletal muscle protein, antigen standard for HSPB1 (transient overexpression lysate), mouse skeletal muscle protein.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha B-crystallin; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha 2; crystallin, alpha B; epididymis secretory protein Li 101; Heat shock protein beta-5; heat shock protein CryAB; Heat shock protein family B member 5; heat shock protein HspB5; heat-shock 20 kD like-protein; HspB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; P23
UniProt ID: (Human) P02511, (Rat) P23928, (Mouse) P23927
Entrez Gene ID: (Human) 1410, (Rat) 25420, (Mouse) 12955
response to hypoxia
lens development in camera-type eye
protein folding
muscle contraction
tubulin complex assembly
muscle organ development
aging
negative regulation of gene expression
regulation of cell death
negative regulation of cell growth
microtubule polymerization or depolymerization
response to estradiol
negative regulation of intracellular transport
response to hydrogen peroxide
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein homooligomerization
stress-activated MAPK cascade
apoptotic process involved in morphogenesis
cellular response to gamma radiation
regulation of cellular response to heat
negative regulation of reactive oxygen species metabolic process
camera-type eye development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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